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High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma
BACKGROUND: Cancer represents a serious threat for human health with high social and economic impacts worldwide. Therefore, the development of new anticancer drugs is of most importance. The aim of the present study was to evaluate the antitumor potential of twelve algae from Portugal coast on an in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987755/ https://www.ncbi.nlm.nih.gov/pubmed/27588232 http://dx.doi.org/10.1186/s40064-016-2938-2 |
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author | Alves, Celso Pinteus, Susete Horta, André Pedrosa, Rui |
author_facet | Alves, Celso Pinteus, Susete Horta, André Pedrosa, Rui |
author_sort | Alves, Celso |
collection | PubMed |
description | BACKGROUND: Cancer represents a serious threat for human health with high social and economic impacts worldwide. Therefore, the development of new anticancer drugs is of most importance. The aim of the present study was to evaluate the antitumor potential of twelve algae from Portugal coast on an in vitro model of human hepatocellular carcinoma (HepG-2 cells). RESULTS: Both extracts of Asparagopsis armata (1000 µg/ml; 24 h) presented high cytotoxicity with 11.22 ± 2.98 and 1.51 ± 0.38 % of HepG-2 live cells, respectively. Sphaerococcus coronopifolius methanolic and dichloromethane extracts (1000 µg/ml) also generated high reduction on HepG-2 viability (14.04 ± 2.62 and 12.84 ± 3.82 % of HepG-2 live cells, respectively). The most potent anti-proliferative activity was induced by dichloromethane extract (1000 µg/ml; 24 h) of Sphaerococcus coronopifolius, Asparagopsis armata and Plocamium cartilagineum with 99.61 ± 0.27, 98.56 ± 0.81 and 85.13 ± 1.04 % of cell’s proliferation reduction, respectively. Sphaerococcus coronopifolius dichloromethane extract exhibited the highest potency both on cytotoxicity and anti-proliferation assays with an IC(50) of 14.1 and 32.3 μg/ml, respectively. CONCLUSIONS: Sphaerococcus coronopifolius is a promising source of new molecules with possible application on cancer therapeutics. |
format | Online Article Text |
id | pubmed-4987755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-49877552016-09-01 High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma Alves, Celso Pinteus, Susete Horta, André Pedrosa, Rui Springerplus Research BACKGROUND: Cancer represents a serious threat for human health with high social and economic impacts worldwide. Therefore, the development of new anticancer drugs is of most importance. The aim of the present study was to evaluate the antitumor potential of twelve algae from Portugal coast on an in vitro model of human hepatocellular carcinoma (HepG-2 cells). RESULTS: Both extracts of Asparagopsis armata (1000 µg/ml; 24 h) presented high cytotoxicity with 11.22 ± 2.98 and 1.51 ± 0.38 % of HepG-2 live cells, respectively. Sphaerococcus coronopifolius methanolic and dichloromethane extracts (1000 µg/ml) also generated high reduction on HepG-2 viability (14.04 ± 2.62 and 12.84 ± 3.82 % of HepG-2 live cells, respectively). The most potent anti-proliferative activity was induced by dichloromethane extract (1000 µg/ml; 24 h) of Sphaerococcus coronopifolius, Asparagopsis armata and Plocamium cartilagineum with 99.61 ± 0.27, 98.56 ± 0.81 and 85.13 ± 1.04 % of cell’s proliferation reduction, respectively. Sphaerococcus coronopifolius dichloromethane extract exhibited the highest potency both on cytotoxicity and anti-proliferation assays with an IC(50) of 14.1 and 32.3 μg/ml, respectively. CONCLUSIONS: Sphaerococcus coronopifolius is a promising source of new molecules with possible application on cancer therapeutics. Springer International Publishing 2016-08-12 /pmc/articles/PMC4987755/ /pubmed/27588232 http://dx.doi.org/10.1186/s40064-016-2938-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Alves, Celso Pinteus, Susete Horta, André Pedrosa, Rui High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title | High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title_full | High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title_fullStr | High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title_full_unstemmed | High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title_short | High cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
title_sort | high cytotoxicity and anti-proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987755/ https://www.ncbi.nlm.nih.gov/pubmed/27588232 http://dx.doi.org/10.1186/s40064-016-2938-2 |
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