Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance

BACKGROUND: Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis, which results in economic and welfare costs in the poultry industry worldwide. The pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the function of an outer membrane protein encoded by the ir...

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Autores principales: Li, Yaxin, Dai, Jianjun, Zhuge, Xiangkai, Wang, Haojin, Hu, Lin, Ren, Jianluan, Chen, Ling, Li, Dezhi, Tang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988017/
https://www.ncbi.nlm.nih.gov/pubmed/27531140
http://dx.doi.org/10.1186/s12917-016-0800-y
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author Li, Yaxin
Dai, Jianjun
Zhuge, Xiangkai
Wang, Haojin
Hu, Lin
Ren, Jianluan
Chen, Ling
Li, Dezhi
Tang, Fang
author_facet Li, Yaxin
Dai, Jianjun
Zhuge, Xiangkai
Wang, Haojin
Hu, Lin
Ren, Jianluan
Chen, Ling
Li, Dezhi
Tang, Fang
author_sort Li, Yaxin
collection PubMed
description BACKGROUND: Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis, which results in economic and welfare costs in the poultry industry worldwide. The pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the function of an outer membrane protein encoded by the ireA gene of avian pathogenic E. coli strain DE205B was investigated. RESULTS: The ireA gene was distributed in 32.9 % (46/140) of tested E. coli strains, with high percentages in the phylogenetic ECOR groups B2 (58.8 %, 10/17) and D (55.9 %, 19/34). The gene expression level of ireA of APEC strain DE205B in high Fe M9 media was 1.8 times higher (P < 0.05) than that in low Fe M9 media. An ireA deletion mutant and complementary strain were constructed. Compared with the wild-type strain DE205B, the expression of most ferric uptake genes in the ireA deletion mutant were significantly upregulated (P < 0.05). The adhesion ability of the ireA deletion mutant to DF-1 cells was significantly decreased. The survival rate of ireA deletion mutant was reduced 21.17 % (P < 0.01), 25.42 (P < 0.05) and 70.0 % (P < 0.01) under alkali, high osmolarity, and low temperature (4 °C) conditions, respectively, compared with the wild-type strain. CONCLUSIONS: The results suggested that the protein encoded by the iron-regulated gene ireA has roles in adhesion and stress resistance in avian pathogenic E. coli. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-016-0800-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-49880172016-08-18 Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance Li, Yaxin Dai, Jianjun Zhuge, Xiangkai Wang, Haojin Hu, Lin Ren, Jianluan Chen, Ling Li, Dezhi Tang, Fang BMC Vet Res Research Article BACKGROUND: Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis, which results in economic and welfare costs in the poultry industry worldwide. The pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the function of an outer membrane protein encoded by the ireA gene of avian pathogenic E. coli strain DE205B was investigated. RESULTS: The ireA gene was distributed in 32.9 % (46/140) of tested E. coli strains, with high percentages in the phylogenetic ECOR groups B2 (58.8 %, 10/17) and D (55.9 %, 19/34). The gene expression level of ireA of APEC strain DE205B in high Fe M9 media was 1.8 times higher (P < 0.05) than that in low Fe M9 media. An ireA deletion mutant and complementary strain were constructed. Compared with the wild-type strain DE205B, the expression of most ferric uptake genes in the ireA deletion mutant were significantly upregulated (P < 0.05). The adhesion ability of the ireA deletion mutant to DF-1 cells was significantly decreased. The survival rate of ireA deletion mutant was reduced 21.17 % (P < 0.01), 25.42 (P < 0.05) and 70.0 % (P < 0.01) under alkali, high osmolarity, and low temperature (4 °C) conditions, respectively, compared with the wild-type strain. CONCLUSIONS: The results suggested that the protein encoded by the iron-regulated gene ireA has roles in adhesion and stress resistance in avian pathogenic E. coli. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-016-0800-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-08-17 /pmc/articles/PMC4988017/ /pubmed/27531140 http://dx.doi.org/10.1186/s12917-016-0800-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Yaxin
Dai, Jianjun
Zhuge, Xiangkai
Wang, Haojin
Hu, Lin
Ren, Jianluan
Chen, Ling
Li, Dezhi
Tang, Fang
Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title_full Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title_fullStr Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title_full_unstemmed Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title_short Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance
title_sort iron-regulated gene irea in avian pathogenic escherichia coli participates in adhesion and stress-resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988017/
https://www.ncbi.nlm.nih.gov/pubmed/27531140
http://dx.doi.org/10.1186/s12917-016-0800-y
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