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The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences

Previous results suggested that the gastric function plays a paramount role in digestive efficiency differences between D+ and D− broiler lines divergently selected for AME(n) (more feed efficient and less feed efficient, respectively). In this paper we show an histological analysis of the gastric i...

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Autores principales: Rideau, N., Godet, E., Combémorel, C., Chaudeau, M., Carré, B., Mignon-Grasteau, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Poultry Science Association, Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988555/
https://www.ncbi.nlm.nih.gov/pubmed/24795318
http://dx.doi.org/10.3382/ps.2013-03756
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author Rideau, N.
Godet, E.
Combémorel, C.
Chaudeau, M.
Carré, B.
Mignon-Grasteau, S.
author_facet Rideau, N.
Godet, E.
Combémorel, C.
Chaudeau, M.
Carré, B.
Mignon-Grasteau, S.
author_sort Rideau, N.
collection PubMed
description Previous results suggested that the gastric function plays a paramount role in digestive efficiency differences between D+ and D− broiler lines divergently selected for AME(n) (more feed efficient and less feed efficient, respectively). In this paper we show an histological analysis of the gastric isthmus located between the proventriculus and the gizzard in the D+ and D− lines. Cross-sections were performed using a cryostat (Leica CM30505) and stained with a routine procedure using Mayer’s Hematoxylin and Eosin Stain. The surface and shape of the constitutive gastric isthmus tissues were quantified using the image analysis software Image J. The lumen occupied 11% of the whole D− isthmus cross-sectional area against 24% for D+ (P < 0.01). The mucosa relative area (cm(2)/total cross-sectional area) was higher in D− than in D+ [47% (D−) and 39% (D+), P < 0.01]. It was significantly more oval and more folded on the lumen side in D− than in D+ chickens; the muscle layer (muscularis mucosae) of the mucosa was relatively more developed in D− than in D+ (16 and 11% of the section, respectively; P < 0.01). A relationship between these observations and increased gastric motility reported in D− compared with D+ is discussed.
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spelling pubmed-49885552016-09-01 The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences Rideau, N. Godet, E. Combémorel, C. Chaudeau, M. Carré, B. Mignon-Grasteau, S. Poult Sci Physiology, Endocrinology, and Reproduction Previous results suggested that the gastric function plays a paramount role in digestive efficiency differences between D+ and D− broiler lines divergently selected for AME(n) (more feed efficient and less feed efficient, respectively). In this paper we show an histological analysis of the gastric isthmus located between the proventriculus and the gizzard in the D+ and D− lines. Cross-sections were performed using a cryostat (Leica CM30505) and stained with a routine procedure using Mayer’s Hematoxylin and Eosin Stain. The surface and shape of the constitutive gastric isthmus tissues were quantified using the image analysis software Image J. The lumen occupied 11% of the whole D− isthmus cross-sectional area against 24% for D+ (P < 0.01). The mucosa relative area (cm(2)/total cross-sectional area) was higher in D− than in D+ [47% (D−) and 39% (D+), P < 0.01]. It was significantly more oval and more folded on the lumen side in D− than in D+ chickens; the muscle layer (muscularis mucosae) of the mucosa was relatively more developed in D− than in D+ (16 and 11% of the section, respectively; P < 0.01). A relationship between these observations and increased gastric motility reported in D− compared with D+ is discussed. Poultry Science Association, Inc. 2014-05 2014-04-28 /pmc/articles/PMC4988555/ /pubmed/24795318 http://dx.doi.org/10.3382/ps.2013-03756 Text en © 2014 Poultry Science Association Inc. This article is available free under the terms of the journal's open access policy http://creativecommons.org/licenses/by/3.0/
spellingShingle Physiology, Endocrinology, and Reproduction
Rideau, N.
Godet, E.
Combémorel, C.
Chaudeau, M.
Carré, B.
Mignon-Grasteau, S.
The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title_full The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title_fullStr The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title_full_unstemmed The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title_short The gastric isthmus from D+ and D− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
title_sort gastric isthmus from d+ and d− broiler lines divergently selected for digestion efficiency shows histological and morphological differences
topic Physiology, Endocrinology, and Reproduction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988555/
https://www.ncbi.nlm.nih.gov/pubmed/24795318
http://dx.doi.org/10.3382/ps.2013-03756
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