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Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle

Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to in...

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Autores principales: Diniz, Wellison Jarles da Silva, Coutinho, Luiz Lehmann, Tizioto, Polyana Cristine, Cesar, Aline Silva Mello, Gromboni, Caio Fernando, Nogueira, Ana Rita Araújo, de Oliveira, Priscila Silva Neubern, de Souza, Marcela Maria, Regitano, Luciana Correia de Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988672/
https://www.ncbi.nlm.nih.gov/pubmed/27532424
http://dx.doi.org/10.1371/journal.pone.0161160
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author Diniz, Wellison Jarles da Silva
Coutinho, Luiz Lehmann
Tizioto, Polyana Cristine
Cesar, Aline Silva Mello
Gromboni, Caio Fernando
Nogueira, Ana Rita Araújo
de Oliveira, Priscila Silva Neubern
de Souza, Marcela Maria
Regitano, Luciana Correia de Almeida
author_facet Diniz, Wellison Jarles da Silva
Coutinho, Luiz Lehmann
Tizioto, Polyana Cristine
Cesar, Aline Silva Mello
Gromboni, Caio Fernando
Nogueira, Ana Rita Araújo
de Oliveira, Priscila Silva Neubern
de Souza, Marcela Maria
Regitano, Luciana Correia de Almeida
author_sort Diniz, Wellison Jarles da Silva
collection PubMed
description Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were up-regulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology.
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spelling pubmed-49886722016-08-29 Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle Diniz, Wellison Jarles da Silva Coutinho, Luiz Lehmann Tizioto, Polyana Cristine Cesar, Aline Silva Mello Gromboni, Caio Fernando Nogueira, Ana Rita Araújo de Oliveira, Priscila Silva Neubern de Souza, Marcela Maria Regitano, Luciana Correia de Almeida PLoS One Research Article Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were up-regulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology. Public Library of Science 2016-08-17 /pmc/articles/PMC4988672/ /pubmed/27532424 http://dx.doi.org/10.1371/journal.pone.0161160 Text en © 2016 Diniz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Diniz, Wellison Jarles da Silva
Coutinho, Luiz Lehmann
Tizioto, Polyana Cristine
Cesar, Aline Silva Mello
Gromboni, Caio Fernando
Nogueira, Ana Rita Araújo
de Oliveira, Priscila Silva Neubern
de Souza, Marcela Maria
Regitano, Luciana Correia de Almeida
Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title_full Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title_fullStr Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title_full_unstemmed Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title_short Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle
title_sort iron content affects lipogenic gene expression in the muscle of nelore beef cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988672/
https://www.ncbi.nlm.nih.gov/pubmed/27532424
http://dx.doi.org/10.1371/journal.pone.0161160
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