The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing

The RNA-guided Cas9 nuclease, from the type II prokaryotic Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) adaptive immune system, has been adapted and utilized by scientists to edit the genomes of eukaryotic cells. Here, we report the development of a viral mediated CRISPR/Cas9...

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Detalles Bibliográficos
Autores principales: de Solis, Christopher A., Ho, Anthony, Holehonnur, Roopashri, Ploski, Jonathan E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988984/
https://www.ncbi.nlm.nih.gov/pubmed/27587996
http://dx.doi.org/10.3389/fnmol.2016.00070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988984/
https://www.ncbi.nlm.nih.gov/pubmed/27587996
http://dx.doi.org/10.3389/fnmol.2016.00070

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