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Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer

Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly high...

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Autores principales: Funston, Scott J., Tsaousi, Konstantina, Rudden, Michelle, Smyth, Thomas J., Stevenson, Paul S., Marchant, Roger, Banat, Ibrahim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989024/
https://www.ncbi.nlm.nih.gov/pubmed/27147528
http://dx.doi.org/10.1007/s00253-016-7564-y
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author Funston, Scott J.
Tsaousi, Konstantina
Rudden, Michelle
Smyth, Thomas J.
Stevenson, Paul S.
Marchant, Roger
Banat, Ibrahim M.
author_facet Funston, Scott J.
Tsaousi, Konstantina
Rudden, Michelle
Smyth, Thomas J.
Stevenson, Paul S.
Marchant, Roger
Banat, Ibrahim M.
author_sort Funston, Scott J.
collection PubMed
description Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture. Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.
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spelling pubmed-49890242016-09-01 Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer Funston, Scott J. Tsaousi, Konstantina Rudden, Michelle Smyth, Thomas J. Stevenson, Paul S. Marchant, Roger Banat, Ibrahim M. Appl Microbiol Biotechnol Biotechnological Products and Process Engineering Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture. Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production. Springer Berlin Heidelberg 2016-05-05 2016 /pmc/articles/PMC4989024/ /pubmed/27147528 http://dx.doi.org/10.1007/s00253-016-7564-y Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Biotechnological Products and Process Engineering
Funston, Scott J.
Tsaousi, Konstantina
Rudden, Michelle
Smyth, Thomas J.
Stevenson, Paul S.
Marchant, Roger
Banat, Ibrahim M.
Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title_full Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title_fullStr Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title_full_unstemmed Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title_short Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer
title_sort characterising rhamnolipid production in burkholderia thailandensis e264, a non-pathogenic producer
topic Biotechnological Products and Process Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989024/
https://www.ncbi.nlm.nih.gov/pubmed/27147528
http://dx.doi.org/10.1007/s00253-016-7564-y
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