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Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats

Circulating microRNAs are protected from degradation by their association with either vesicles or components of the RNAi machinery. Although increasing evidence indicates that cell-free microRNAs are transported in body fluids by different types of vesicles, current research mainly focuses on the ch...

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Autores principales: Castoldi, Mirco, Kordes, Claus, Sawitza, Iris, Häussinger, Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989158/
https://www.ncbi.nlm.nih.gov/pubmed/27535708
http://dx.doi.org/10.1038/srep31869
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author Castoldi, Mirco
Kordes, Claus
Sawitza, Iris
Häussinger, Dieter
author_facet Castoldi, Mirco
Kordes, Claus
Sawitza, Iris
Häussinger, Dieter
author_sort Castoldi, Mirco
collection PubMed
description Circulating microRNAs are protected from degradation by their association with either vesicles or components of the RNAi machinery. Although increasing evidence indicates that cell-free microRNAs are transported in body fluids by different types of vesicles, current research mainly focuses on the characterization of exosome-associated microRNAs. However, as isolation and characterization of exosomes is challenging, it is yet unclear whether exosomes or other vesicular elements circulating in serum are the most reliable source for discovering disease-associated biomarkers. In this study, circulating microRNAs associated to the vesicular and non-vesicular fraction of sera isolated from partially hepatectomized rats were measured. Here we show that independently from their origin, levels of miR-122, miR-192, miR-194 and Let-7a are up-regulated two days after partial hepatectomy. The inflammation-associated miR-150 and miR-155 are up-regulated in the vesicular-fraction only, while the regeneration-associated miR-21 and miR-33 are up-regulated in the vesicular- and down-regulated in the non-vesicular fraction. Our study shows for the first time the modulation of non-vesicular microRNAs in animals recovering from partial hepatectomy, suggesting that, in the search for novel disease-associated biomarkers, the profiling of either vesicular or non-vesicular microRNAs may be more relevant than the analysis of microRNAs isolated from unfractionated serum.
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spelling pubmed-49891582016-08-30 Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats Castoldi, Mirco Kordes, Claus Sawitza, Iris Häussinger, Dieter Sci Rep Article Circulating microRNAs are protected from degradation by their association with either vesicles or components of the RNAi machinery. Although increasing evidence indicates that cell-free microRNAs are transported in body fluids by different types of vesicles, current research mainly focuses on the characterization of exosome-associated microRNAs. However, as isolation and characterization of exosomes is challenging, it is yet unclear whether exosomes or other vesicular elements circulating in serum are the most reliable source for discovering disease-associated biomarkers. In this study, circulating microRNAs associated to the vesicular and non-vesicular fraction of sera isolated from partially hepatectomized rats were measured. Here we show that independently from their origin, levels of miR-122, miR-192, miR-194 and Let-7a are up-regulated two days after partial hepatectomy. The inflammation-associated miR-150 and miR-155 are up-regulated in the vesicular-fraction only, while the regeneration-associated miR-21 and miR-33 are up-regulated in the vesicular- and down-regulated in the non-vesicular fraction. Our study shows for the first time the modulation of non-vesicular microRNAs in animals recovering from partial hepatectomy, suggesting that, in the search for novel disease-associated biomarkers, the profiling of either vesicular or non-vesicular microRNAs may be more relevant than the analysis of microRNAs isolated from unfractionated serum. Nature Publishing Group 2016-08-18 /pmc/articles/PMC4989158/ /pubmed/27535708 http://dx.doi.org/10.1038/srep31869 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Castoldi, Mirco
Kordes, Claus
Sawitza, Iris
Häussinger, Dieter
Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title_full Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title_fullStr Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title_full_unstemmed Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title_short Isolation and characterization of vesicular and non-vesicular microRNAs circulating in sera of partially hepatectomized rats
title_sort isolation and characterization of vesicular and non-vesicular micrornas circulating in sera of partially hepatectomized rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989158/
https://www.ncbi.nlm.nih.gov/pubmed/27535708
http://dx.doi.org/10.1038/srep31869
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