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Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488
The relBE family of Type II toxin-antitoxin (TA) systems have been widely reported in bacteria but none in Streptomyces. With the conserved domain searches for TA pairs in the sequenced Streptomyces genomes, we identified two putative relBE loci, relBE1sca and relBE2sca, on the chromosome of Strepto...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989188/ https://www.ncbi.nlm.nih.gov/pubmed/27534445 http://dx.doi.org/10.1038/srep32047 |
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author | Li, Peng Tai, Cui Deng, Zixin Gan, Jianhua Oggioni, Marco R. Ou, Hong-Yu |
author_facet | Li, Peng Tai, Cui Deng, Zixin Gan, Jianhua Oggioni, Marco R. Ou, Hong-Yu |
author_sort | Li, Peng |
collection | PubMed |
description | The relBE family of Type II toxin-antitoxin (TA) systems have been widely reported in bacteria but none in Streptomyces. With the conserved domain searches for TA pairs in the sequenced Streptomyces genomes, we identified two putative relBE loci, relBE1sca and relBE2sca, on the chromosome of Streptomyces cattleya DSM 46488. Overexpression of the S. cattleya toxin RelE2sca caused severe growth inhibition of E. coli and S. lividans, but RelE1sca had no toxic effect. The toxicity of RelE2sca could be abolished by the co-expression of its cognate RelB2sca antitoxin. Moreover, the RelBE2sca complex, or the antitoxin RelB2sca alone, specifically interacted with the relBE2sca operon and repressed its transcription. The relBE2sca operon transcription was induced under osmotic stress, along with the ClpP proteinase genes. The subsequent in vivo analysis showed that the antitoxin was degraded by ClpP. Interestingly, the E. coli antitoxin RelBeco was able to alleviate the toxicity of S. cattleya RelE2sca while the mutant RelB2sca(N61V&M68L) but not the wild type could alleviate the toxicity of E. coli RelEeco as well. The experimental demonstration of the relBEsca locus might be helpful to investigate the key roles of type II TA systems in Streptomyces physiology and environmental stress responses. |
format | Online Article Text |
id | pubmed-4989188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49891882016-08-30 Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 Li, Peng Tai, Cui Deng, Zixin Gan, Jianhua Oggioni, Marco R. Ou, Hong-Yu Sci Rep Article The relBE family of Type II toxin-antitoxin (TA) systems have been widely reported in bacteria but none in Streptomyces. With the conserved domain searches for TA pairs in the sequenced Streptomyces genomes, we identified two putative relBE loci, relBE1sca and relBE2sca, on the chromosome of Streptomyces cattleya DSM 46488. Overexpression of the S. cattleya toxin RelE2sca caused severe growth inhibition of E. coli and S. lividans, but RelE1sca had no toxic effect. The toxicity of RelE2sca could be abolished by the co-expression of its cognate RelB2sca antitoxin. Moreover, the RelBE2sca complex, or the antitoxin RelB2sca alone, specifically interacted with the relBE2sca operon and repressed its transcription. The relBE2sca operon transcription was induced under osmotic stress, along with the ClpP proteinase genes. The subsequent in vivo analysis showed that the antitoxin was degraded by ClpP. Interestingly, the E. coli antitoxin RelBeco was able to alleviate the toxicity of S. cattleya RelE2sca while the mutant RelB2sca(N61V&M68L) but not the wild type could alleviate the toxicity of E. coli RelEeco as well. The experimental demonstration of the relBEsca locus might be helpful to investigate the key roles of type II TA systems in Streptomyces physiology and environmental stress responses. Nature Publishing Group 2016-08-18 /pmc/articles/PMC4989188/ /pubmed/27534445 http://dx.doi.org/10.1038/srep32047 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Li, Peng Tai, Cui Deng, Zixin Gan, Jianhua Oggioni, Marco R. Ou, Hong-Yu Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title | Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title_full | Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title_fullStr | Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title_full_unstemmed | Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title_short | Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488 |
title_sort | identification and characterization of chromosomal relbe toxin-antitoxin locus in streptomyces cattleya dsm46488 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989188/ https://www.ncbi.nlm.nih.gov/pubmed/27534445 http://dx.doi.org/10.1038/srep32047 |
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