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Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis

The bilateral common carotid artery stenosis (BCAS) mouse model, which replicates chronic cerebral hypoperfusion and white matter ischemic lesions, is considered to model some aspects of vascular cognitive impairment. Cerebral blood flow (CBF) changes in the brain surface post-BCAS have been demonst...

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Autores principales: Hattori, Yorito, Enmi, Jun-ichiro, Iguchi, Satoshi, Saito, Satoshi, Yamamoto, Yumi, Nagatsuka, Kazuyuki, Iida, Hidehiro, Ihara, Masafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989493/
https://www.ncbi.nlm.nih.gov/pubmed/27535801
http://dx.doi.org/10.1038/srep32179
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author Hattori, Yorito
Enmi, Jun-ichiro
Iguchi, Satoshi
Saito, Satoshi
Yamamoto, Yumi
Nagatsuka, Kazuyuki
Iida, Hidehiro
Ihara, Masafumi
author_facet Hattori, Yorito
Enmi, Jun-ichiro
Iguchi, Satoshi
Saito, Satoshi
Yamamoto, Yumi
Nagatsuka, Kazuyuki
Iida, Hidehiro
Ihara, Masafumi
author_sort Hattori, Yorito
collection PubMed
description The bilateral common carotid artery stenosis (BCAS) mouse model, which replicates chronic cerebral hypoperfusion and white matter ischemic lesions, is considered to model some aspects of vascular cognitive impairment. Cerebral blood flow (CBF) changes in the brain surface post-BCAS have been demonstrated by laser speckle flowmetry, but CBF levels in the brain parenchyma remain unknown. Adult C57BL/6J male mice were subjected to BCAS using external microcoils. Brain magnetic resonance angiography (MRA) was conducted to visualize the intracranial main arteries while arterial spin labeling (ASL) was used to measure cortical and subcortical parenchymal CBF levels before and after BCAS. Brain MRA showed anterior circulation flow was substantially decreased until 14 days post-BCAS, which gradually but incompletely recovered over the following 14 days, with probable growth of collaterals from the posterior cerebral artery. ASL showed that cortical and subcortical parenchymal CBF remained decreased at approximately 50% of the baseline level during 1 and 14 days post-BCAS, recovering to approximately 70% at day 28. CBF levels in the parenchyma were lower than the cortical superficial region in the BCAS model and remained decreased without recovery during the first 2 weeks post-BCAS. These results suggest that the BCAS model reliably replicates chronic cerebral hypoperfusion.
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spelling pubmed-49894932016-08-30 Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis Hattori, Yorito Enmi, Jun-ichiro Iguchi, Satoshi Saito, Satoshi Yamamoto, Yumi Nagatsuka, Kazuyuki Iida, Hidehiro Ihara, Masafumi Sci Rep Article The bilateral common carotid artery stenosis (BCAS) mouse model, which replicates chronic cerebral hypoperfusion and white matter ischemic lesions, is considered to model some aspects of vascular cognitive impairment. Cerebral blood flow (CBF) changes in the brain surface post-BCAS have been demonstrated by laser speckle flowmetry, but CBF levels in the brain parenchyma remain unknown. Adult C57BL/6J male mice were subjected to BCAS using external microcoils. Brain magnetic resonance angiography (MRA) was conducted to visualize the intracranial main arteries while arterial spin labeling (ASL) was used to measure cortical and subcortical parenchymal CBF levels before and after BCAS. Brain MRA showed anterior circulation flow was substantially decreased until 14 days post-BCAS, which gradually but incompletely recovered over the following 14 days, with probable growth of collaterals from the posterior cerebral artery. ASL showed that cortical and subcortical parenchymal CBF remained decreased at approximately 50% of the baseline level during 1 and 14 days post-BCAS, recovering to approximately 70% at day 28. CBF levels in the parenchyma were lower than the cortical superficial region in the BCAS model and remained decreased without recovery during the first 2 weeks post-BCAS. These results suggest that the BCAS model reliably replicates chronic cerebral hypoperfusion. Nature Publishing Group 2016-08-18 /pmc/articles/PMC4989493/ /pubmed/27535801 http://dx.doi.org/10.1038/srep32179 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hattori, Yorito
Enmi, Jun-ichiro
Iguchi, Satoshi
Saito, Satoshi
Yamamoto, Yumi
Nagatsuka, Kazuyuki
Iida, Hidehiro
Ihara, Masafumi
Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title_full Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title_fullStr Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title_full_unstemmed Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title_short Substantial Reduction of Parenchymal Cerebral Blood Flow in Mice with Bilateral Common Carotid Artery Stenosis
title_sort substantial reduction of parenchymal cerebral blood flow in mice with bilateral common carotid artery stenosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989493/
https://www.ncbi.nlm.nih.gov/pubmed/27535801
http://dx.doi.org/10.1038/srep32179
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