A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines

More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase...

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Detalles Bibliográficos
Autores principales: Ogura, Yuji, Parsons, William H., Kamat, Siddhesh S., Cravatt, Benjamin F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4990470/
https://www.ncbi.nlm.nih.gov/pubmed/27399000
http://dx.doi.org/10.1038/nchembio.2127
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author Ogura, Yuji
Parsons, William H.
Kamat, Siddhesh S.
Cravatt, Benjamin F.
author_facet Ogura, Yuji
Parsons, William H.
Kamat, Siddhesh S.
Cravatt, Benjamin F.
author_sort Ogura, Yuji
collection PubMed
description More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.
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spelling pubmed-49904702017-01-11 A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines Ogura, Yuji Parsons, William H. Kamat, Siddhesh S. Cravatt, Benjamin F. Nat Chem Biol Article More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells. 2016-07-11 2016-09 /pmc/articles/PMC4990470/ /pubmed/27399000 http://dx.doi.org/10.1038/nchembio.2127 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ogura, Yuji
Parsons, William H.
Kamat, Siddhesh S.
Cravatt, Benjamin F.
A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title_full A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title_fullStr A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title_full_unstemmed A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title_short A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
title_sort calcium-dependent acyltransferase that produces n-acyl phosphatidylethanolamines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4990470/
https://www.ncbi.nlm.nih.gov/pubmed/27399000
http://dx.doi.org/10.1038/nchembio.2127
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