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Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia

The aim of the present study was to examine and understand changes in platelet functions prior to and after the treatment of primary immune thrombocytopenia (ITP) in children. An automatic hematology analyzer and whole blood flow cytometry were used to detect immature platelet fraction (IPF), IPC an...

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Autores principales: Liu, Wen-Jun, Bai, Jing, Guo, Qu-Lian, Huang, Zhe, Yang, Hong, Bai, Yong-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991683/
https://www.ncbi.nlm.nih.gov/pubmed/27431926
http://dx.doi.org/10.3892/mmr.2016.5504
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author Liu, Wen-Jun
Bai, Jing
Guo, Qu-Lian
Huang, Zhe
Yang, Hong
Bai, Yong-Qi
author_facet Liu, Wen-Jun
Bai, Jing
Guo, Qu-Lian
Huang, Zhe
Yang, Hong
Bai, Yong-Qi
author_sort Liu, Wen-Jun
collection PubMed
description The aim of the present study was to examine and understand changes in platelet functions prior to and after the treatment of primary immune thrombocytopenia (ITP) in children. An automatic hematology analyzer and whole blood flow cytometry were used to detect immature platelet fraction (IPF), IPC and membrane glycoproteins (CD62p, PAC-1 and CD42b) in ITP children (ITP group), children with complete response after ITP treatment (ITP-CR group) and children with elective surgery (normal control group). The results showed that, levels of platelet count (PLT) and plateletcrit in the ITP group were lower alhtough the levels of mean platelet volume, platelet distribution width and platelet-large cell ratio (P-LCR) were higher than those in the normal control and ITP-CR groups. PLT in the ITP-CR group was lower than that in the normal controls. Additionally, IPF% was higher in the normal control and ITP-CR groups, IPC was lower in the ITP group compared to the normal control and ITP-CR groups. Furthermore, prior to ADP activation, the expression levels of CD62p, PAC-1 and CD42b in the ITP group were lower in ITP group than those in the normal control and ITP-CR groups. The expression level of PAC-1 was lower in the ITP-CR and normal control groups. No differences were identified in CD62p and CD42b expression levels. Following ATP activation, CD62p, PAC-1 and CD42b expression in the ITP group was lower than that in the normal control and ITP-CR groups. PAC-1 expression was lower while CD62p expression was higher in the ITP-CR group compared to the normal control group. In conclusion, the activation of platelets in ITP children was low. Decreased platelet function, platelet parameters and platelet glycoproteins may be used as markers for monitoring the treatment efficacy in ITP children.
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spelling pubmed-49916832016-08-26 Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia Liu, Wen-Jun Bai, Jing Guo, Qu-Lian Huang, Zhe Yang, Hong Bai, Yong-Qi Mol Med Rep Articles The aim of the present study was to examine and understand changes in platelet functions prior to and after the treatment of primary immune thrombocytopenia (ITP) in children. An automatic hematology analyzer and whole blood flow cytometry were used to detect immature platelet fraction (IPF), IPC and membrane glycoproteins (CD62p, PAC-1 and CD42b) in ITP children (ITP group), children with complete response after ITP treatment (ITP-CR group) and children with elective surgery (normal control group). The results showed that, levels of platelet count (PLT) and plateletcrit in the ITP group were lower alhtough the levels of mean platelet volume, platelet distribution width and platelet-large cell ratio (P-LCR) were higher than those in the normal control and ITP-CR groups. PLT in the ITP-CR group was lower than that in the normal controls. Additionally, IPF% was higher in the normal control and ITP-CR groups, IPC was lower in the ITP group compared to the normal control and ITP-CR groups. Furthermore, prior to ADP activation, the expression levels of CD62p, PAC-1 and CD42b in the ITP group were lower in ITP group than those in the normal control and ITP-CR groups. The expression level of PAC-1 was lower in the ITP-CR and normal control groups. No differences were identified in CD62p and CD42b expression levels. Following ATP activation, CD62p, PAC-1 and CD42b expression in the ITP group was lower than that in the normal control and ITP-CR groups. PAC-1 expression was lower while CD62p expression was higher in the ITP-CR group compared to the normal control group. In conclusion, the activation of platelets in ITP children was low. Decreased platelet function, platelet parameters and platelet glycoproteins may be used as markers for monitoring the treatment efficacy in ITP children. D.A. Spandidos 2016-09 2016-07-11 /pmc/articles/PMC4991683/ /pubmed/27431926 http://dx.doi.org/10.3892/mmr.2016.5504 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Wen-Jun
Bai, Jing
Guo, Qu-Lian
Huang, Zhe
Yang, Hong
Bai, Yong-Qi
Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title_full Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title_fullStr Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title_full_unstemmed Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title_short Role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
title_sort role of platelet function and platelet membrane glycoproteins in children with primary immune thrombocytopenia
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991683/
https://www.ncbi.nlm.nih.gov/pubmed/27431926
http://dx.doi.org/10.3892/mmr.2016.5504
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