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High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster

The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which...

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Autores principales: Trindade de Paula, Mariane, Poetini Silva, Márcia Rósula, Machado Araujo, Stífani, Cardoso Bortolotto, Vandreza, Barreto Meichtry, Luana, Zemolin, Ana Paula Pegoraro, Wallau, Gabriel L., Jesse, Cristiano Ricardo, Franco, Jeferson Luís, Posser, Thaís, Prigol, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992541/
https://www.ncbi.nlm.nih.gov/pubmed/27579152
http://dx.doi.org/10.1155/2016/4018157
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author Trindade de Paula, Mariane
Poetini Silva, Márcia Rósula
Machado Araujo, Stífani
Cardoso Bortolotto, Vandreza
Barreto Meichtry, Luana
Zemolin, Ana Paula Pegoraro
Wallau, Gabriel L.
Jesse, Cristiano Ricardo
Franco, Jeferson Luís
Posser, Thaís
Prigol, Marina
author_facet Trindade de Paula, Mariane
Poetini Silva, Márcia Rósula
Machado Araujo, Stífani
Cardoso Bortolotto, Vandreza
Barreto Meichtry, Luana
Zemolin, Ana Paula Pegoraro
Wallau, Gabriel L.
Jesse, Cristiano Ricardo
Franco, Jeferson Luís
Posser, Thaís
Prigol, Marina
author_sort Trindade de Paula, Mariane
collection PubMed
description The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.
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spelling pubmed-49925412016-08-30 High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster Trindade de Paula, Mariane Poetini Silva, Márcia Rósula Machado Araujo, Stífani Cardoso Bortolotto, Vandreza Barreto Meichtry, Luana Zemolin, Ana Paula Pegoraro Wallau, Gabriel L. Jesse, Cristiano Ricardo Franco, Jeferson Luís Posser, Thaís Prigol, Marina Oxid Med Cell Longev Research Article The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD. Hindawi Publishing Corporation 2016 2016-08-07 /pmc/articles/PMC4992541/ /pubmed/27579152 http://dx.doi.org/10.1155/2016/4018157 Text en Copyright © 2016 Mariane Trindade de Paula et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Trindade de Paula, Mariane
Poetini Silva, Márcia Rósula
Machado Araujo, Stífani
Cardoso Bortolotto, Vandreza
Barreto Meichtry, Luana
Zemolin, Ana Paula Pegoraro
Wallau, Gabriel L.
Jesse, Cristiano Ricardo
Franco, Jeferson Luís
Posser, Thaís
Prigol, Marina
High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title_full High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title_fullStr High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title_full_unstemmed High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title_short High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
title_sort high-fat diet induces oxidative stress and mpk2 and hsp83 gene expression in drosophila melanogaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992541/
https://www.ncbi.nlm.nih.gov/pubmed/27579152
http://dx.doi.org/10.1155/2016/4018157
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