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Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood

Background: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with di...

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Autores principales: Ameer, Syeda S., Xu, YiYi, Engström, Karin, Li, Huiqi, Tallving, Pia, Nermell, Barbro, Boemo, Analia, Parada, Luis A., Peñaloza, Lidia G., Concha, Gabriela, Harari, Florencia, Vahter, Marie, Broberg, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992680/
https://www.ncbi.nlm.nih.gov/pubmed/27597942
http://dx.doi.org/10.3389/fcell.2016.00087
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author Ameer, Syeda S.
Xu, YiYi
Engström, Karin
Li, Huiqi
Tallving, Pia
Nermell, Barbro
Boemo, Analia
Parada, Luis A.
Peñaloza, Lidia G.
Concha, Gabriela
Harari, Florencia
Vahter, Marie
Broberg, Karin
author_facet Ameer, Syeda S.
Xu, YiYi
Engström, Karin
Li, Huiqi
Tallving, Pia
Nermell, Barbro
Boemo, Analia
Parada, Luis A.
Peñaloza, Lidia G.
Concha, Gabriela
Harari, Florencia
Vahter, Marie
Broberg, Karin
author_sort Ameer, Syeda S.
collection PubMed
description Background: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with different arsenic metabolizing capacity. Methods: We studied two groups in the Salta province, Argentina, one in the Puna area of the Andes (N = 264, 89% females) and one in Chaco (N = 169, 75% females). We assessed arsenic exposure as the sum of arsenic metabolites [iAs, methylarsonic acid (MMA), dimethylarsinic acid (DMA)] in urine (U-As) using high-performance liquid chromatography coupled with hydride generation and inductively coupled plasma mass spectrometry. Efficiency of arsenic metabolism was expressed as percentage of urinary metabolites. MtDNAcn and telomere length were determined in blood by real-time PCR. Results: Median U-As was 196 (5–95 percentile: 21–537) μg/L in Andes and 80 (5–95 percentile: 15–1637) μg/L in Chaco. The latter study group had less-efficient metabolism, with higher %iAs and %MMA in urine compared with the Andean group. U-As was significantly associated with increased mtDNAcn (log2 transformed to improve linearity) in Chaco (β = 0.027 per 100 μg/L, p = 0.0085; adjusted for age and sex), but not in Andes (β = 0.025, p = 0.24). U-As was also associated with longer telomere length in Chaco (β = 0.016, p = 0.0066) and Andes (β = 0.0075, p = 0.029). In both populations, individuals with above median %iAs showed significantly higher mtDNAcn and telomere length compared with individuals with below median %iAs. Conclusions: Arsenic was associated with increased mtDNAcn and telomere length, particularly in individuals with less-efficient arsenic metabolism, a group who may have increased risk for arsenic-related cancer.
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spelling pubmed-49926802016-09-05 Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood Ameer, Syeda S. Xu, YiYi Engström, Karin Li, Huiqi Tallving, Pia Nermell, Barbro Boemo, Analia Parada, Luis A. Peñaloza, Lidia G. Concha, Gabriela Harari, Florencia Vahter, Marie Broberg, Karin Front Cell Dev Biol Cell and Developmental Biology Background: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with different arsenic metabolizing capacity. Methods: We studied two groups in the Salta province, Argentina, one in the Puna area of the Andes (N = 264, 89% females) and one in Chaco (N = 169, 75% females). We assessed arsenic exposure as the sum of arsenic metabolites [iAs, methylarsonic acid (MMA), dimethylarsinic acid (DMA)] in urine (U-As) using high-performance liquid chromatography coupled with hydride generation and inductively coupled plasma mass spectrometry. Efficiency of arsenic metabolism was expressed as percentage of urinary metabolites. MtDNAcn and telomere length were determined in blood by real-time PCR. Results: Median U-As was 196 (5–95 percentile: 21–537) μg/L in Andes and 80 (5–95 percentile: 15–1637) μg/L in Chaco. The latter study group had less-efficient metabolism, with higher %iAs and %MMA in urine compared with the Andean group. U-As was significantly associated with increased mtDNAcn (log2 transformed to improve linearity) in Chaco (β = 0.027 per 100 μg/L, p = 0.0085; adjusted for age and sex), but not in Andes (β = 0.025, p = 0.24). U-As was also associated with longer telomere length in Chaco (β = 0.016, p = 0.0066) and Andes (β = 0.0075, p = 0.029). In both populations, individuals with above median %iAs showed significantly higher mtDNAcn and telomere length compared with individuals with below median %iAs. Conclusions: Arsenic was associated with increased mtDNAcn and telomere length, particularly in individuals with less-efficient arsenic metabolism, a group who may have increased risk for arsenic-related cancer. Frontiers Media S.A. 2016-08-22 /pmc/articles/PMC4992680/ /pubmed/27597942 http://dx.doi.org/10.3389/fcell.2016.00087 Text en Copyright © 2016 Ameer, Xu, Engström, Li, Tallving, Nermell, Boemo, Parada, Peñaloza, Concha, Harari, Vahter and Broberg. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ameer, Syeda S.
Xu, YiYi
Engström, Karin
Li, Huiqi
Tallving, Pia
Nermell, Barbro
Boemo, Analia
Parada, Luis A.
Peñaloza, Lidia G.
Concha, Gabriela
Harari, Florencia
Vahter, Marie
Broberg, Karin
Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title_full Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title_fullStr Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title_full_unstemmed Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title_short Exposure to Inorganic Arsenic Is Associated with Increased Mitochondrial DNA Copy Number and Longer Telomere Length in Peripheral Blood
title_sort exposure to inorganic arsenic is associated with increased mitochondrial dna copy number and longer telomere length in peripheral blood
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992680/
https://www.ncbi.nlm.nih.gov/pubmed/27597942
http://dx.doi.org/10.3389/fcell.2016.00087
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