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Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging
Sickle cell disease (SCD) is common across Sub-Saharan Africa. However, the investigation of SCD in this area has been significantly limited mainly due to the lack of research facilities and skilled personnel. Here, we present optical measurements of individual red blood cells from healthy individua...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992839/ https://www.ncbi.nlm.nih.gov/pubmed/27546097 http://dx.doi.org/10.1038/srep31698 |
_version_ | 1782449063661142016 |
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author | Jung, JaeHwang Matemba, Lucas E. Lee, KyeoReh Kazyoba, Paul E. Yoon, Jonghee Massaga, Julius J. Kim, Kyoohyun Kim, Dong-Jin Park, YongKeun |
author_facet | Jung, JaeHwang Matemba, Lucas E. Lee, KyeoReh Kazyoba, Paul E. Yoon, Jonghee Massaga, Julius J. Kim, Kyoohyun Kim, Dong-Jin Park, YongKeun |
author_sort | Jung, JaeHwang |
collection | PubMed |
description | Sickle cell disease (SCD) is common across Sub-Saharan Africa. However, the investigation of SCD in this area has been significantly limited mainly due to the lack of research facilities and skilled personnel. Here, we present optical measurements of individual red blood cells from healthy individuals and individuals with SCD and sickle cell trait in Tanzania using the quantitative phase imaging technique. By employing a quantitative phase imaging unit, an existing microscope in a clinic is transformed into a powerful quantitative phase microscope providing measurements on the morphological, biochemical, and biomechanical properties of individual cells. The present approach will open up new opportunities for cost-effective investigation and diagnosis of several diseases in low resource environments. |
format | Online Article Text |
id | pubmed-4992839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49928392016-08-30 Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging Jung, JaeHwang Matemba, Lucas E. Lee, KyeoReh Kazyoba, Paul E. Yoon, Jonghee Massaga, Julius J. Kim, Kyoohyun Kim, Dong-Jin Park, YongKeun Sci Rep Article Sickle cell disease (SCD) is common across Sub-Saharan Africa. However, the investigation of SCD in this area has been significantly limited mainly due to the lack of research facilities and skilled personnel. Here, we present optical measurements of individual red blood cells from healthy individuals and individuals with SCD and sickle cell trait in Tanzania using the quantitative phase imaging technique. By employing a quantitative phase imaging unit, an existing microscope in a clinic is transformed into a powerful quantitative phase microscope providing measurements on the morphological, biochemical, and biomechanical properties of individual cells. The present approach will open up new opportunities for cost-effective investigation and diagnosis of several diseases in low resource environments. Nature Publishing Group 2016-08-22 /pmc/articles/PMC4992839/ /pubmed/27546097 http://dx.doi.org/10.1038/srep31698 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jung, JaeHwang Matemba, Lucas E. Lee, KyeoReh Kazyoba, Paul E. Yoon, Jonghee Massaga, Julius J. Kim, Kyoohyun Kim, Dong-Jin Park, YongKeun Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title | Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title_full | Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title_fullStr | Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title_full_unstemmed | Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title_short | Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging |
title_sort | optical characterization of red blood cells from individuals with sickle cell trait and disease in tanzania using quantitative phase imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992839/ https://www.ncbi.nlm.nih.gov/pubmed/27546097 http://dx.doi.org/10.1038/srep31698 |
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