Cargando…
Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models
Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is b...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993383/ https://www.ncbi.nlm.nih.gov/pubmed/27501019 http://dx.doi.org/10.3791/53609 |
_version_ | 1782449142378790912 |
---|---|
author | Cosette, Jeremie Ben Abdelwahed, Rym Donnou-Triffault, Sabrina Sautès-Fridman, Catherine Flaud, Patrice Fisson, Sylvain |
author_facet | Cosette, Jeremie Ben Abdelwahed, Rym Donnou-Triffault, Sabrina Sautès-Fridman, Catherine Flaud, Patrice Fisson, Sylvain |
author_sort | Cosette, Jeremie |
collection | PubMed |
description | Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse). Using two previously described mouse lymphoma models based on cell engraftment, we show that the luminoscore method can serve as a noninvasive way to verify successful tumor cell inoculation, monitor tumor burden, and evaluate the effects of in situ cancer treatment (CpG-DNA). Finally, we show that this method suits different experimental designs. We suggest that this method be used for early estimates of treatment response in preclinical small-animal studies. |
format | Online Article Text |
id | pubmed-4993383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49933832016-09-20 Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models Cosette, Jeremie Ben Abdelwahed, Rym Donnou-Triffault, Sabrina Sautès-Fridman, Catherine Flaud, Patrice Fisson, Sylvain J Vis Exp Medicine Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse). Using two previously described mouse lymphoma models based on cell engraftment, we show that the luminoscore method can serve as a noninvasive way to verify successful tumor cell inoculation, monitor tumor burden, and evaluate the effects of in situ cancer treatment (CpG-DNA). Finally, we show that this method suits different experimental designs. We suggest that this method be used for early estimates of treatment response in preclinical small-animal studies. MyJove Corporation 2016-07-07 /pmc/articles/PMC4993383/ /pubmed/27501019 http://dx.doi.org/10.3791/53609 Text en Copyright © 2016, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Medicine Cosette, Jeremie Ben Abdelwahed, Rym Donnou-Triffault, Sabrina Sautès-Fridman, Catherine Flaud, Patrice Fisson, Sylvain Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title | Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title_full | Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title_fullStr | Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title_full_unstemmed | Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title_short | Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models |
title_sort | bioluminescence-based tumor quantification method for monitoring tumor progression and treatment effects in mouse lymphoma models |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993383/ https://www.ncbi.nlm.nih.gov/pubmed/27501019 http://dx.doi.org/10.3791/53609 |
work_keys_str_mv | AT cosettejeremie bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels AT benabdelwahedrym bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels AT donnoutriffaultsabrina bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels AT sautesfridmancatherine bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels AT flaudpatrice bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels AT fissonsylvain bioluminescencebasedtumorquantificationmethodformonitoringtumorprogressionandtreatmenteffectsinmouselymphomamodels |