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Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii
The inner membrane complex (IMC) of Toxoplasma gondii as a peripheral membrane system has unique and critical roles in parasite replication, motility and invasion. Disruption of IMC sub-compartment protein produces a severe defect in T. gondii endodyogeny, the form of internal cell budding. In this...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993474/ https://www.ncbi.nlm.nih.gov/pubmed/27548677 http://dx.doi.org/10.1371/journal.pone.0161231 |
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author | Lee, Dong Hun Lee, Su Hwa Kim, Ah Ra Quan, Fu Shi |
author_facet | Lee, Dong Hun Lee, Su Hwa Kim, Ah Ra Quan, Fu Shi |
author_sort | Lee, Dong Hun |
collection | PubMed |
description | The inner membrane complex (IMC) of Toxoplasma gondii as a peripheral membrane system has unique and critical roles in parasite replication, motility and invasion. Disruption of IMC sub-compartment protein produces a severe defect in T. gondii endodyogeny, the form of internal cell budding. In this study, we generated T. gondii virus-like particle particles (VLPs) containing proteins derived from IMC, and investigated their efficacy as a vaccine in mice. VLP vaccination induced Toxoplasma gondii-specific total IgG, IgG1 and IgG2a antibody responses in the sera and IgA antibody responses in the feces. Upon challenge infection with a lethal dose of T. gondii (ME49), all vaccinated mice survived, whereas all naïve control mice died. Vaccinated mice showed significantly reduced cyst load and cyst size in the brain. VLP vaccination also induced IgA and IgG antibody responses in feces and intestines, and antibody-secreting plasma cells, mixed Th1/Th2 cytokines and CD4(+)/CD8(+) T cells from spleen. Taken together, these results indicate that non-replicating VLPs containing inner membrane complex of T. gondii represent a promising strategy for the development of a safe and effective vaccine to control the spread of Toxoplasma gondii infection. |
format | Online Article Text |
id | pubmed-4993474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49934742016-09-12 Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii Lee, Dong Hun Lee, Su Hwa Kim, Ah Ra Quan, Fu Shi PLoS One Research Article The inner membrane complex (IMC) of Toxoplasma gondii as a peripheral membrane system has unique and critical roles in parasite replication, motility and invasion. Disruption of IMC sub-compartment protein produces a severe defect in T. gondii endodyogeny, the form of internal cell budding. In this study, we generated T. gondii virus-like particle particles (VLPs) containing proteins derived from IMC, and investigated their efficacy as a vaccine in mice. VLP vaccination induced Toxoplasma gondii-specific total IgG, IgG1 and IgG2a antibody responses in the sera and IgA antibody responses in the feces. Upon challenge infection with a lethal dose of T. gondii (ME49), all vaccinated mice survived, whereas all naïve control mice died. Vaccinated mice showed significantly reduced cyst load and cyst size in the brain. VLP vaccination also induced IgA and IgG antibody responses in feces and intestines, and antibody-secreting plasma cells, mixed Th1/Th2 cytokines and CD4(+)/CD8(+) T cells from spleen. Taken together, these results indicate that non-replicating VLPs containing inner membrane complex of T. gondii represent a promising strategy for the development of a safe and effective vaccine to control the spread of Toxoplasma gondii infection. Public Library of Science 2016-08-22 /pmc/articles/PMC4993474/ /pubmed/27548677 http://dx.doi.org/10.1371/journal.pone.0161231 Text en © 2016 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lee, Dong Hun Lee, Su Hwa Kim, Ah Ra Quan, Fu Shi Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title | Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title_full | Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title_fullStr | Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title_full_unstemmed | Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title_short | Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii |
title_sort | virus-like nanoparticle vaccine confers protection against toxoplasma gondii |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993474/ https://www.ncbi.nlm.nih.gov/pubmed/27548677 http://dx.doi.org/10.1371/journal.pone.0161231 |
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