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A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria

Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate...

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Autores principales: Yuan, Meng, Ke, Yinggen, Huang, Renyan, Ma, Ling, Yang, Zeyu, Chu, Zhaohui, Xiao, Jinghua, Li, Xianghua, Wang, Shiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993585/
https://www.ncbi.nlm.nih.gov/pubmed/27472897
http://dx.doi.org/10.7554/eLife.19605
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author Yuan, Meng
Ke, Yinggen
Huang, Renyan
Ma, Ling
Yang, Zeyu
Chu, Zhaohui
Xiao, Jinghua
Li, Xianghua
Wang, Shiping
author_facet Yuan, Meng
Ke, Yinggen
Huang, Renyan
Ma, Ling
Yang, Zeyu
Chu, Zhaohui
Xiao, Jinghua
Li, Xianghua
Wang, Shiping
author_sort Yuan, Meng
collection PubMed
description Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens. DOI: http://dx.doi.org/10.7554/eLife.19605.001
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spelling pubmed-49935852016-08-24 A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria Yuan, Meng Ke, Yinggen Huang, Renyan Ma, Ling Yang, Zeyu Chu, Zhaohui Xiao, Jinghua Li, Xianghua Wang, Shiping eLife Plant Biology Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens. DOI: http://dx.doi.org/10.7554/eLife.19605.001 eLife Sciences Publications, Ltd 2016-07-29 /pmc/articles/PMC4993585/ /pubmed/27472897 http://dx.doi.org/10.7554/eLife.19605 Text en © 2016, Yuan et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Yuan, Meng
Ke, Yinggen
Huang, Renyan
Ma, Ling
Yang, Zeyu
Chu, Zhaohui
Xiao, Jinghua
Li, Xianghua
Wang, Shiping
A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title_full A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title_fullStr A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title_full_unstemmed A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title_short A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
title_sort host basal transcription factor is a key component for infection of rice by tale-carrying bacteria
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993585/
https://www.ncbi.nlm.nih.gov/pubmed/27472897
http://dx.doi.org/10.7554/eLife.19605
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