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Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993730/ https://www.ncbi.nlm.nih.gov/pubmed/27610292 http://dx.doi.org/10.1186/s40064-016-2902-1 |
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author | Zhu, Long Chen, Tao Sui, Menghua Han, Chunyang Fang, Fugui Ma, Yuehui Chu, Mingxing Zhang, Xiaorong Liu, Cuiyan Ling, Yinghui |
author_facet | Zhu, Long Chen, Tao Sui, Menghua Han, Chunyang Fang, Fugui Ma, Yuehui Chu, Mingxing Zhang, Xiaorong Liu, Cuiyan Ling, Yinghui |
author_sort | Zhu, Long |
collection | PubMed |
description | To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2902-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4993730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-49937302016-09-08 Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats Zhu, Long Chen, Tao Sui, Menghua Han, Chunyang Fang, Fugui Ma, Yuehui Chu, Mingxing Zhang, Xiaorong Liu, Cuiyan Ling, Yinghui Springerplus Research To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2902-1) contains supplementary material, which is available to authorized users. Springer International Publishing 2016-08-02 /pmc/articles/PMC4993730/ /pubmed/27610292 http://dx.doi.org/10.1186/s40064-016-2902-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Zhu, Long Chen, Tao Sui, Menghua Han, Chunyang Fang, Fugui Ma, Yuehui Chu, Mingxing Zhang, Xiaorong Liu, Cuiyan Ling, Yinghui Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title | Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title_full | Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title_fullStr | Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title_full_unstemmed | Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title_short | Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats |
title_sort | comparative profiling of differentially expressed micrornas between the follicular and luteal phases ovaries of goats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993730/ https://www.ncbi.nlm.nih.gov/pubmed/27610292 http://dx.doi.org/10.1186/s40064-016-2902-1 |
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