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Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats

To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total,...

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Autores principales: Zhu, Long, Chen, Tao, Sui, Menghua, Han, Chunyang, Fang, Fugui, Ma, Yuehui, Chu, Mingxing, Zhang, Xiaorong, Liu, Cuiyan, Ling, Yinghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993730/
https://www.ncbi.nlm.nih.gov/pubmed/27610292
http://dx.doi.org/10.1186/s40064-016-2902-1
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author Zhu, Long
Chen, Tao
Sui, Menghua
Han, Chunyang
Fang, Fugui
Ma, Yuehui
Chu, Mingxing
Zhang, Xiaorong
Liu, Cuiyan
Ling, Yinghui
author_facet Zhu, Long
Chen, Tao
Sui, Menghua
Han, Chunyang
Fang, Fugui
Ma, Yuehui
Chu, Mingxing
Zhang, Xiaorong
Liu, Cuiyan
Ling, Yinghui
author_sort Zhu, Long
collection PubMed
description To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2902-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-49937302016-09-08 Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats Zhu, Long Chen, Tao Sui, Menghua Han, Chunyang Fang, Fugui Ma, Yuehui Chu, Mingxing Zhang, Xiaorong Liu, Cuiyan Ling, Yinghui Springerplus Research To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2902-1) contains supplementary material, which is available to authorized users. Springer International Publishing 2016-08-02 /pmc/articles/PMC4993730/ /pubmed/27610292 http://dx.doi.org/10.1186/s40064-016-2902-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Zhu, Long
Chen, Tao
Sui, Menghua
Han, Chunyang
Fang, Fugui
Ma, Yuehui
Chu, Mingxing
Zhang, Xiaorong
Liu, Cuiyan
Ling, Yinghui
Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title_full Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title_fullStr Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title_full_unstemmed Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title_short Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats
title_sort comparative profiling of differentially expressed micrornas between the follicular and luteal phases ovaries of goats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993730/
https://www.ncbi.nlm.nih.gov/pubmed/27610292
http://dx.doi.org/10.1186/s40064-016-2902-1
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