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Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato

Plant hormones have become appropriate candidates for driving functional plant mycorrhization programs, including the processes that regulate the formation of arbuscules in arbuscular mycorrhizal (AM) symbiosis. Here, we examine the role played by ABA/GA interactions regulating the formation of AM i...

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Autores principales: Martín-Rodríguez, José A., Huertas, Raúl, Ho-Plágaro, Tania, Ocampo, Juan A., Turečková, Veronika, Tarkowská, Danuše, Ludwig-Müller, Jutta, García-Garrido, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993810/
https://www.ncbi.nlm.nih.gov/pubmed/27602046
http://dx.doi.org/10.3389/fpls.2016.01273
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author Martín-Rodríguez, José A.
Huertas, Raúl
Ho-Plágaro, Tania
Ocampo, Juan A.
Turečková, Veronika
Tarkowská, Danuše
Ludwig-Müller, Jutta
García-Garrido, José M.
author_facet Martín-Rodríguez, José A.
Huertas, Raúl
Ho-Plágaro, Tania
Ocampo, Juan A.
Turečková, Veronika
Tarkowská, Danuše
Ludwig-Müller, Jutta
García-Garrido, José M.
author_sort Martín-Rodríguez, José A.
collection PubMed
description Plant hormones have become appropriate candidates for driving functional plant mycorrhization programs, including the processes that regulate the formation of arbuscules in arbuscular mycorrhizal (AM) symbiosis. Here, we examine the role played by ABA/GA interactions regulating the formation of AM in tomato. We report differences in ABA and GA metabolism between control and mycorrhizal roots. Active synthesis and catabolism of ABA occur in AM roots. GAs level increases as a consequence of a symbiosis-induced mechanism that requires functional arbuscules which in turn is dependent on a functional ABA pathway. A negative interaction in their metabolism has been demonstrated. ABA attenuates GA-biosynthetic and increases GA-catabolic gene expression leading to a reduction in bioactive GAs. Vice versa, GA activated ABA catabolism mainly in mycorrhizal roots. The negative impact of GA(3) on arbuscule abundance in wild-type plants is partially offset by treatment with ABA and the application of a GA biosynthesis inhibitor rescued the arbuscule abundance in the ABA-deficient sitiens mutant. These findings, coupled with the evidence that ABA application leads to reduce bioactive GA(1), support the hypothesis that ABA could act modifying bioactive GA level to regulate AM. Taken together, our results suggest that these hormones perform essential functions and antagonize each other by oppositely regulating AM formation in tomato roots.
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spelling pubmed-49938102016-09-06 Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato Martín-Rodríguez, José A. Huertas, Raúl Ho-Plágaro, Tania Ocampo, Juan A. Turečková, Veronika Tarkowská, Danuše Ludwig-Müller, Jutta García-Garrido, José M. Front Plant Sci Plant Science Plant hormones have become appropriate candidates for driving functional plant mycorrhization programs, including the processes that regulate the formation of arbuscules in arbuscular mycorrhizal (AM) symbiosis. Here, we examine the role played by ABA/GA interactions regulating the formation of AM in tomato. We report differences in ABA and GA metabolism between control and mycorrhizal roots. Active synthesis and catabolism of ABA occur in AM roots. GAs level increases as a consequence of a symbiosis-induced mechanism that requires functional arbuscules which in turn is dependent on a functional ABA pathway. A negative interaction in their metabolism has been demonstrated. ABA attenuates GA-biosynthetic and increases GA-catabolic gene expression leading to a reduction in bioactive GAs. Vice versa, GA activated ABA catabolism mainly in mycorrhizal roots. The negative impact of GA(3) on arbuscule abundance in wild-type plants is partially offset by treatment with ABA and the application of a GA biosynthesis inhibitor rescued the arbuscule abundance in the ABA-deficient sitiens mutant. These findings, coupled with the evidence that ABA application leads to reduce bioactive GA(1), support the hypothesis that ABA could act modifying bioactive GA level to regulate AM. Taken together, our results suggest that these hormones perform essential functions and antagonize each other by oppositely regulating AM formation in tomato roots. Frontiers Media S.A. 2016-08-23 /pmc/articles/PMC4993810/ /pubmed/27602046 http://dx.doi.org/10.3389/fpls.2016.01273 Text en Copyright © 2016 Martín-Rodríguez, Huertas, Ho-Plágaro, Ocampo, Turečková, Tarkowská, Ludwig-Müller and García-Garrido. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Martín-Rodríguez, José A.
Huertas, Raúl
Ho-Plágaro, Tania
Ocampo, Juan A.
Turečková, Veronika
Tarkowská, Danuše
Ludwig-Müller, Jutta
García-Garrido, José M.
Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title_full Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title_fullStr Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title_full_unstemmed Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title_short Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato
title_sort gibberellin–abscisic acid balances during arbuscular mycorrhiza formation in tomato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993810/
https://www.ncbi.nlm.nih.gov/pubmed/27602046
http://dx.doi.org/10.3389/fpls.2016.01273
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