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Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients

BACKGROUND AND AIMS: Several angiogenic factors are involved in the development and progression of hepatocellular carcinoma (HCC), a hypervascular tumor. Vascular endothelial growth factor (VEGF) is a primary driving force for angiogenesis, and its overexpression has been reported in HCC. However, t...

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Autores principales: Atta, Mohamed Magdi El-Sadek Ali, Atta, Hazem Mahmoud, Gad, Magdy Abdel-Mawgoud, Rashed, Laila Ahmad, Said, Ebada M, Hassanien, Sharaf El-Sayed Ali, Kaseb, Ahmed O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994801/
https://www.ncbi.nlm.nih.gov/pubmed/27574588
http://dx.doi.org/10.2147/JHC.S86708
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author Atta, Mohamed Magdi El-Sadek Ali
Atta, Hazem Mahmoud
Gad, Magdy Abdel-Mawgoud
Rashed, Laila Ahmad
Said, Ebada M
Hassanien, Sharaf El-Sayed Ali
Kaseb, Ahmed O
author_facet Atta, Mohamed Magdi El-Sadek Ali
Atta, Hazem Mahmoud
Gad, Magdy Abdel-Mawgoud
Rashed, Laila Ahmad
Said, Ebada M
Hassanien, Sharaf El-Sayed Ali
Kaseb, Ahmed O
author_sort Atta, Mohamed Magdi El-Sadek Ali
collection PubMed
description BACKGROUND AND AIMS: Several angiogenic factors are involved in the development and progression of hepatocellular carcinoma (HCC), a hypervascular tumor. Vascular endothelial growth factor (VEGF) is a primary driving force for angiogenesis, and its overexpression has been reported in HCC. However, the significance of plasma and tissue VEGF levels in HCC in Egyptian patients with chronic hepatitis C (CHC) infection is understudied. The aim of this study was to evaluate the role of VEGF (measured in plasma and liver tissue) in patients with hepatitis C virus-related HCC and to assess its significance in the diagnosis and prognosis of HCC. MATERIALS AND METHODS: A total of 90 subjects were studied. Among 90 subjects, 60 with CHC were examined and were subdivided into two groups: 30 patients with CHC-related HCC (HCC group) and 30 patients with CHC without HCC (non-HCC group). Thirty apparently healthy subjects served as the control group. VEGF was estimated in plasma by enzyme-linked immunosorbent assay and its expression in liver tissue was evaluated by real-time polymerase chain reaction. VEGF expression level and its relationship to tumor parameters, patients’ liver function profile, and patients’ clinical parameters were also investigated. RESULTS: Plasma VEGF levels in the HCC group were significantly higher than those of the non-HCC group, and both groups had significantly higher plasma VEGF levels than did the control group. Liver tissue VEGF expression was significantly higher in the HCC group than in the non-HCC group and positively correlated with plasma VEGF in the HCC group. The plasma VEGF levels were positively correlated with patients’ age, aspartate aminotransferase levels, serum alpha-fetoprotein levels, the presence of portal vein thrombosis, and the number of hepatic focal lesions in the HCC group. However, plasma VEGF levels were not significantly correlated with the Child-Pugh score, alanine aminotransferase levels, the size of focal lesions, and Okuda stage. Using both the VEGF and alpha-fetoprotein levels to detect HCC maximizes the sensitivity and specificity. CONCLUSION: Plasma levels of VEGF may be a useful diagnostic and prognostic marker for HCC in patients who have been diagnosed with CHC.
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spelling pubmed-49948012016-08-29 Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients Atta, Mohamed Magdi El-Sadek Ali Atta, Hazem Mahmoud Gad, Magdy Abdel-Mawgoud Rashed, Laila Ahmad Said, Ebada M Hassanien, Sharaf El-Sayed Ali Kaseb, Ahmed O J Hepatocell Carcinoma Original Research BACKGROUND AND AIMS: Several angiogenic factors are involved in the development and progression of hepatocellular carcinoma (HCC), a hypervascular tumor. Vascular endothelial growth factor (VEGF) is a primary driving force for angiogenesis, and its overexpression has been reported in HCC. However, the significance of plasma and tissue VEGF levels in HCC in Egyptian patients with chronic hepatitis C (CHC) infection is understudied. The aim of this study was to evaluate the role of VEGF (measured in plasma and liver tissue) in patients with hepatitis C virus-related HCC and to assess its significance in the diagnosis and prognosis of HCC. MATERIALS AND METHODS: A total of 90 subjects were studied. Among 90 subjects, 60 with CHC were examined and were subdivided into two groups: 30 patients with CHC-related HCC (HCC group) and 30 patients with CHC without HCC (non-HCC group). Thirty apparently healthy subjects served as the control group. VEGF was estimated in plasma by enzyme-linked immunosorbent assay and its expression in liver tissue was evaluated by real-time polymerase chain reaction. VEGF expression level and its relationship to tumor parameters, patients’ liver function profile, and patients’ clinical parameters were also investigated. RESULTS: Plasma VEGF levels in the HCC group were significantly higher than those of the non-HCC group, and both groups had significantly higher plasma VEGF levels than did the control group. Liver tissue VEGF expression was significantly higher in the HCC group than in the non-HCC group and positively correlated with plasma VEGF in the HCC group. The plasma VEGF levels were positively correlated with patients’ age, aspartate aminotransferase levels, serum alpha-fetoprotein levels, the presence of portal vein thrombosis, and the number of hepatic focal lesions in the HCC group. However, plasma VEGF levels were not significantly correlated with the Child-Pugh score, alanine aminotransferase levels, the size of focal lesions, and Okuda stage. Using both the VEGF and alpha-fetoprotein levels to detect HCC maximizes the sensitivity and specificity. CONCLUSION: Plasma levels of VEGF may be a useful diagnostic and prognostic marker for HCC in patients who have been diagnosed with CHC. Dove Medical Press 2016-06-14 /pmc/articles/PMC4994801/ /pubmed/27574588 http://dx.doi.org/10.2147/JHC.S86708 Text en © 2016 Atta et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Atta, Mohamed Magdi El-Sadek Ali
Atta, Hazem Mahmoud
Gad, Magdy Abdel-Mawgoud
Rashed, Laila Ahmad
Said, Ebada M
Hassanien, Sharaf El-Sayed Ali
Kaseb, Ahmed O
Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title_full Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title_fullStr Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title_full_unstemmed Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title_short Clinical significance of vascular endothelial growth factor in hepatitis C related hepatocellular carcinoma in Egyptian patients
title_sort clinical significance of vascular endothelial growth factor in hepatitis c related hepatocellular carcinoma in egyptian patients
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994801/
https://www.ncbi.nlm.nih.gov/pubmed/27574588
http://dx.doi.org/10.2147/JHC.S86708
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