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Nitrogen fixation and nifH diversity in human gut microbiota

It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on...

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Autores principales: Igai, Katsura, Itakura, Manabu, Nishijima, Suguru, Tsurumaru, Hirohito, Suda, Wataru, Tsutaya, Takumi, Tomitsuka, Eriko, Tadokoro, Kiyoshi, Baba, Jun, Odani, Shingo, Natsuhara, Kazumi, Morita, Ayako, Yoneda, Minoru, Greenhill, Andrew R., Horwood, Paul F., Inoue, Jun-ichi, Ohkuma, Moriya, Hongoh, Yuichi, Yamamoto, Taro, Siba, Peter M., Hattori, Masahira, Minamisawa, Kiwamu, Umezaki, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995403/
https://www.ncbi.nlm.nih.gov/pubmed/27554344
http://dx.doi.org/10.1038/srep31942
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author Igai, Katsura
Itakura, Manabu
Nishijima, Suguru
Tsurumaru, Hirohito
Suda, Wataru
Tsutaya, Takumi
Tomitsuka, Eriko
Tadokoro, Kiyoshi
Baba, Jun
Odani, Shingo
Natsuhara, Kazumi
Morita, Ayako
Yoneda, Minoru
Greenhill, Andrew R.
Horwood, Paul F.
Inoue, Jun-ichi
Ohkuma, Moriya
Hongoh, Yuichi
Yamamoto, Taro
Siba, Peter M.
Hattori, Masahira
Minamisawa, Kiwamu
Umezaki, Masahiro
author_facet Igai, Katsura
Itakura, Manabu
Nishijima, Suguru
Tsurumaru, Hirohito
Suda, Wataru
Tsutaya, Takumi
Tomitsuka, Eriko
Tadokoro, Kiyoshi
Baba, Jun
Odani, Shingo
Natsuhara, Kazumi
Morita, Ayako
Yoneda, Minoru
Greenhill, Andrew R.
Horwood, Paul F.
Inoue, Jun-ichi
Ohkuma, Moriya
Hongoh, Yuichi
Yamamoto, Taro
Siba, Peter M.
Hattori, Masahira
Minamisawa, Kiwamu
Umezaki, Masahiro
author_sort Igai, Katsura
collection PubMed
description It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A (15)N(2) incorporation assay showed significant enrichment of (15)N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut in vivo might be higher than this value. The nifH genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to Klebsiella sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to Klebsiella and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance.
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spelling pubmed-49954032016-08-30 Nitrogen fixation and nifH diversity in human gut microbiota Igai, Katsura Itakura, Manabu Nishijima, Suguru Tsurumaru, Hirohito Suda, Wataru Tsutaya, Takumi Tomitsuka, Eriko Tadokoro, Kiyoshi Baba, Jun Odani, Shingo Natsuhara, Kazumi Morita, Ayako Yoneda, Minoru Greenhill, Andrew R. Horwood, Paul F. Inoue, Jun-ichi Ohkuma, Moriya Hongoh, Yuichi Yamamoto, Taro Siba, Peter M. Hattori, Masahira Minamisawa, Kiwamu Umezaki, Masahiro Sci Rep Article It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A (15)N(2) incorporation assay showed significant enrichment of (15)N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut in vivo might be higher than this value. The nifH genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to Klebsiella sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to Klebsiella and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance. Nature Publishing Group 2016-08-24 /pmc/articles/PMC4995403/ /pubmed/27554344 http://dx.doi.org/10.1038/srep31942 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Igai, Katsura
Itakura, Manabu
Nishijima, Suguru
Tsurumaru, Hirohito
Suda, Wataru
Tsutaya, Takumi
Tomitsuka, Eriko
Tadokoro, Kiyoshi
Baba, Jun
Odani, Shingo
Natsuhara, Kazumi
Morita, Ayako
Yoneda, Minoru
Greenhill, Andrew R.
Horwood, Paul F.
Inoue, Jun-ichi
Ohkuma, Moriya
Hongoh, Yuichi
Yamamoto, Taro
Siba, Peter M.
Hattori, Masahira
Minamisawa, Kiwamu
Umezaki, Masahiro
Nitrogen fixation and nifH diversity in human gut microbiota
title Nitrogen fixation and nifH diversity in human gut microbiota
title_full Nitrogen fixation and nifH diversity in human gut microbiota
title_fullStr Nitrogen fixation and nifH diversity in human gut microbiota
title_full_unstemmed Nitrogen fixation and nifH diversity in human gut microbiota
title_short Nitrogen fixation and nifH diversity in human gut microbiota
title_sort nitrogen fixation and nifh diversity in human gut microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995403/
https://www.ncbi.nlm.nih.gov/pubmed/27554344
http://dx.doi.org/10.1038/srep31942
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