Cargando…
Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, and...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995462/ https://www.ncbi.nlm.nih.gov/pubmed/27553527 http://dx.doi.org/10.1038/srep32085 |
_version_ | 1782449474391506944 |
---|---|
author | Lin, Po-Han Jian, Cai-Yun Chou, Jou-Chun Chen, Chien-Wei Chen, Chih-Chieh Soong, Christina Hu, Sindy Lieu, Fu-Kong Wang, Paulus S. Wang, Shyi-Wu |
author_facet | Lin, Po-Han Jian, Cai-Yun Chou, Jou-Chun Chen, Chien-Wei Chen, Chih-Chieh Soong, Christina Hu, Sindy Lieu, Fu-Kong Wang, Paulus S. Wang, Shyi-Wu |
author_sort | Lin, Po-Han |
collection | PubMed |
description | The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption. |
format | Online Article Text |
id | pubmed-4995462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49954622016-08-30 Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats Lin, Po-Han Jian, Cai-Yun Chou, Jou-Chun Chen, Chien-Wei Chen, Chih-Chieh Soong, Christina Hu, Sindy Lieu, Fu-Kong Wang, Paulus S. Wang, Shyi-Wu Sci Rep Article The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption. Nature Publishing Group 2016-08-24 /pmc/articles/PMC4995462/ /pubmed/27553527 http://dx.doi.org/10.1038/srep32085 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lin, Po-Han Jian, Cai-Yun Chou, Jou-Chun Chen, Chien-Wei Chen, Chih-Chieh Soong, Christina Hu, Sindy Lieu, Fu-Kong Wang, Paulus S. Wang, Shyi-Wu Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title | Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title_full | Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title_fullStr | Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title_full_unstemmed | Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title_short | Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
title_sort | induction of renal senescence marker protein-30 (smp30) expression by testosterone and its contribution to urinary calcium absorption in male rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995462/ https://www.ncbi.nlm.nih.gov/pubmed/27553527 http://dx.doi.org/10.1038/srep32085 |
work_keys_str_mv | AT linpohan inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT jiancaiyun inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT choujouchun inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT chenchienwei inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT chenchihchieh inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT soongchristina inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT husindy inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT lieufukong inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT wangpauluss inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats AT wangshyiwu inductionofrenalsenescencemarkerprotein30smp30expressionbytestosteroneanditscontributiontourinarycalciumabsorptioninmalerats |