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Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats

The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, and...

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Autores principales: Lin, Po-Han, Jian, Cai-Yun, Chou, Jou-Chun, Chen, Chien-Wei, Chen, Chih-Chieh, Soong, Christina, Hu, Sindy, Lieu, Fu-Kong, Wang, Paulus S., Wang, Shyi-Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995462/
https://www.ncbi.nlm.nih.gov/pubmed/27553527
http://dx.doi.org/10.1038/srep32085
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author Lin, Po-Han
Jian, Cai-Yun
Chou, Jou-Chun
Chen, Chien-Wei
Chen, Chih-Chieh
Soong, Christina
Hu, Sindy
Lieu, Fu-Kong
Wang, Paulus S.
Wang, Shyi-Wu
author_facet Lin, Po-Han
Jian, Cai-Yun
Chou, Jou-Chun
Chen, Chien-Wei
Chen, Chih-Chieh
Soong, Christina
Hu, Sindy
Lieu, Fu-Kong
Wang, Paulus S.
Wang, Shyi-Wu
author_sort Lin, Po-Han
collection PubMed
description The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption.
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spelling pubmed-49954622016-08-30 Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats Lin, Po-Han Jian, Cai-Yun Chou, Jou-Chun Chen, Chien-Wei Chen, Chih-Chieh Soong, Christina Hu, Sindy Lieu, Fu-Kong Wang, Paulus S. Wang, Shyi-Wu Sci Rep Article The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption. Nature Publishing Group 2016-08-24 /pmc/articles/PMC4995462/ /pubmed/27553527 http://dx.doi.org/10.1038/srep32085 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lin, Po-Han
Jian, Cai-Yun
Chou, Jou-Chun
Chen, Chien-Wei
Chen, Chih-Chieh
Soong, Christina
Hu, Sindy
Lieu, Fu-Kong
Wang, Paulus S.
Wang, Shyi-Wu
Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title_full Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title_fullStr Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title_full_unstemmed Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title_short Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats
title_sort induction of renal senescence marker protein-30 (smp30) expression by testosterone and its contribution to urinary calcium absorption in male rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995462/
https://www.ncbi.nlm.nih.gov/pubmed/27553527
http://dx.doi.org/10.1038/srep32085
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