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TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells

Highly Ca(2+) permeable receptor potential channel vanilloid type 6 (TRPV6) modulates a variety of biological functions including calcium-dependent cell growth and apoptosis. So far, the role of TRPV6 in controlling growth of pancreatic neuroendocrine tumour (NET) cells is unknown. In the present st...

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Autores principales: Skrzypski, Marek, Kołodziejski, Paweł A., Mergler, Stefan, Khajavi, Noushafarin, Nowak, Krzysztof W., Strowski, Mathias Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995500/
https://www.ncbi.nlm.nih.gov/pubmed/27450545
http://dx.doi.org/10.1042/BSR20160106
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author Skrzypski, Marek
Kołodziejski, Paweł A.
Mergler, Stefan
Khajavi, Noushafarin
Nowak, Krzysztof W.
Strowski, Mathias Z.
author_facet Skrzypski, Marek
Kołodziejski, Paweł A.
Mergler, Stefan
Khajavi, Noushafarin
Nowak, Krzysztof W.
Strowski, Mathias Z.
author_sort Skrzypski, Marek
collection PubMed
description Highly Ca(2+) permeable receptor potential channel vanilloid type 6 (TRPV6) modulates a variety of biological functions including calcium-dependent cell growth and apoptosis. So far, the role of TRPV6 in controlling growth of pancreatic neuroendocrine tumour (NET) cells is unknown. In the present study, we characterize the expression of TRPV6 in pancreatic BON-1 and QGP-1 NET cells. Furthermore, we evaluate the impact of TRPV6 on intracellular calcium, the activity of nuclear factor of activated T-cells (NFAT) and proliferation of BON-1 cells. TRPV6 expression was assessed by real-time PCR and Western blot. TRPV6 mRNA expression and protein production were down-regulated by siRNA. Changes in intracellular calcium levels were detected by fluorescence calcium imaging (fura-2/AM). NFAT activity was studied by NFAT reporter assay; cell proliferation by bromodeoxyuridine (BrdU), MTT and propidium iodine staining. TRPV6 mRNA and protein are present in BON-1 and QGP-1 NET-cells. Down-regulation of TRPV6 attenuates BON-1 cell proliferation. TRPV6 down-regulation is associated with decreased Ca(2+) response pattern and reduced NFAT activity. In conclusion, TRPV6 is expressed in pancreatic NETs and modulates cell proliferation via Ca(2+)-dependent mechanism, which is accompanied by NFAT activation.
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spelling pubmed-49955002016-09-07 TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells Skrzypski, Marek Kołodziejski, Paweł A. Mergler, Stefan Khajavi, Noushafarin Nowak, Krzysztof W. Strowski, Mathias Z. Biosci Rep Original Papers Highly Ca(2+) permeable receptor potential channel vanilloid type 6 (TRPV6) modulates a variety of biological functions including calcium-dependent cell growth and apoptosis. So far, the role of TRPV6 in controlling growth of pancreatic neuroendocrine tumour (NET) cells is unknown. In the present study, we characterize the expression of TRPV6 in pancreatic BON-1 and QGP-1 NET cells. Furthermore, we evaluate the impact of TRPV6 on intracellular calcium, the activity of nuclear factor of activated T-cells (NFAT) and proliferation of BON-1 cells. TRPV6 expression was assessed by real-time PCR and Western blot. TRPV6 mRNA expression and protein production were down-regulated by siRNA. Changes in intracellular calcium levels were detected by fluorescence calcium imaging (fura-2/AM). NFAT activity was studied by NFAT reporter assay; cell proliferation by bromodeoxyuridine (BrdU), MTT and propidium iodine staining. TRPV6 mRNA and protein are present in BON-1 and QGP-1 NET-cells. Down-regulation of TRPV6 attenuates BON-1 cell proliferation. TRPV6 down-regulation is associated with decreased Ca(2+) response pattern and reduced NFAT activity. In conclusion, TRPV6 is expressed in pancreatic NETs and modulates cell proliferation via Ca(2+)-dependent mechanism, which is accompanied by NFAT activation. Portland Press Ltd. 2016-08-24 /pmc/articles/PMC4995500/ /pubmed/27450545 http://dx.doi.org/10.1042/BSR20160106 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Skrzypski, Marek
Kołodziejski, Paweł A.
Mergler, Stefan
Khajavi, Noushafarin
Nowak, Krzysztof W.
Strowski, Mathias Z.
TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title_full TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title_fullStr TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title_full_unstemmed TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title_short TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour cells
title_sort trpv6 modulates proliferation of human pancreatic neuroendocrine bon-1 tumour cells
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995500/
https://www.ncbi.nlm.nih.gov/pubmed/27450545
http://dx.doi.org/10.1042/BSR20160106
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