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Microintaglio Printing for Soft Lithography-Based in Situ Microarrays

Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored over the last two decades. However, the need for pattern flexibility, a high density, a high resolution, affordability and on-demand fabrication is promoting the development of unconventional routes for...

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Autores principales: Biyani, Manish, Ichiki, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996378/
https://www.ncbi.nlm.nih.gov/pubmed/27600226
http://dx.doi.org/10.3390/microarrays4030311
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author Biyani, Manish
Ichiki, Takanori
author_facet Biyani, Manish
Ichiki, Takanori
author_sort Biyani, Manish
collection PubMed
description Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored over the last two decades. However, the need for pattern flexibility, a high density, a high resolution, affordability and on-demand fabrication is promoting the development of unconventional routes for microarray fabrication. This review highlights the development and uses of a new molecular lithography approach, called “microintaglio printing technology”, for large-scale bio-microarray fabrication using a microreactor array (µRA)-based chip consisting of uniformly-arranged, femtoliter-size µRA molds. In this method, a single-molecule-amplified DNA microarray pattern is self-assembled onto a µRA mold and subsequently converted into a messenger RNA or protein microarray pattern by simultaneously producing and transferring (immobilizing) a messenger RNA or a protein from a µRA mold to a glass surface. Microintaglio printing allows the self-assembly and patterning of in situ-synthesized biomolecules into high-density (kilo-giga-density), ordered arrays on a chip surface with µm-order precision. This holistic aim, which is difficult to achieve using conventional printing and microarray approaches, is expected to revolutionize and reshape proteomics. This review is not written comprehensively, but rather substantively, highlighting the versatility of microintaglio printing for developing a prerequisite platform for microarray technology for the postgenomic era.
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spelling pubmed-49963782016-09-06 Microintaglio Printing for Soft Lithography-Based in Situ Microarrays Biyani, Manish Ichiki, Takanori Microarrays (Basel) Review Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored over the last two decades. However, the need for pattern flexibility, a high density, a high resolution, affordability and on-demand fabrication is promoting the development of unconventional routes for microarray fabrication. This review highlights the development and uses of a new molecular lithography approach, called “microintaglio printing technology”, for large-scale bio-microarray fabrication using a microreactor array (µRA)-based chip consisting of uniformly-arranged, femtoliter-size µRA molds. In this method, a single-molecule-amplified DNA microarray pattern is self-assembled onto a µRA mold and subsequently converted into a messenger RNA or protein microarray pattern by simultaneously producing and transferring (immobilizing) a messenger RNA or a protein from a µRA mold to a glass surface. Microintaglio printing allows the self-assembly and patterning of in situ-synthesized biomolecules into high-density (kilo-giga-density), ordered arrays on a chip surface with µm-order precision. This holistic aim, which is difficult to achieve using conventional printing and microarray approaches, is expected to revolutionize and reshape proteomics. This review is not written comprehensively, but rather substantively, highlighting the versatility of microintaglio printing for developing a prerequisite platform for microarray technology for the postgenomic era. MDPI 2015-07-14 /pmc/articles/PMC4996378/ /pubmed/27600226 http://dx.doi.org/10.3390/microarrays4030311 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Biyani, Manish
Ichiki, Takanori
Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title_full Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title_fullStr Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title_full_unstemmed Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title_short Microintaglio Printing for Soft Lithography-Based in Situ Microarrays
title_sort microintaglio printing for soft lithography-based in situ microarrays
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996378/
https://www.ncbi.nlm.nih.gov/pubmed/27600226
http://dx.doi.org/10.3390/microarrays4030311
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