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Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mamma...

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Autores principales: Larrayoz, Ignacio M., Rey-Funes, Manuel, Contartese, Daniela S., Rolón, Federico, Sarotto, Anibal, Dorfman, Veronica B., Loidl, Cesar F., Martínez, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996528/
https://www.ncbi.nlm.nih.gov/pubmed/27556928
http://dx.doi.org/10.1371/journal.pone.0161458
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author Larrayoz, Ignacio M.
Rey-Funes, Manuel
Contartese, Daniela S.
Rolón, Federico
Sarotto, Anibal
Dorfman, Veronica B.
Loidl, Cesar F.
Martínez, Alfredo
author_facet Larrayoz, Ignacio M.
Rey-Funes, Manuel
Contartese, Daniela S.
Rolón, Federico
Sarotto, Anibal
Dorfman, Veronica B.
Loidl, Cesar F.
Martínez, Alfredo
author_sort Larrayoz, Ignacio M.
collection PubMed
description Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown. Here we show the effects of hypothermia on the expression of these CSPs in retina-derived cell lines and in the retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to a cold environment (8°C) and expression of CSPs in their retinas was studied by Western blotting, multiple inmunofluorescence, and confocal microscopy. RBM3 expression was upregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the other hand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs was negligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposure to a cold environment elicited a strong expression of both proteins, especially in retinal pigment epithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells, and ganglion cells. In conclusion, CSP expression rapidly rises in the mammalian retina following exposure to hypothermia in a cell type-specific pattern. This observation may be at the basis of the molecular mechanism by which hypothermia exerts its therapeutic effects in the retina.
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spelling pubmed-49965282016-09-12 Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures Larrayoz, Ignacio M. Rey-Funes, Manuel Contartese, Daniela S. Rolón, Federico Sarotto, Anibal Dorfman, Veronica B. Loidl, Cesar F. Martínez, Alfredo PLoS One Research Article Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown. Here we show the effects of hypothermia on the expression of these CSPs in retina-derived cell lines and in the retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to a cold environment (8°C) and expression of CSPs in their retinas was studied by Western blotting, multiple inmunofluorescence, and confocal microscopy. RBM3 expression was upregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the other hand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs was negligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposure to a cold environment elicited a strong expression of both proteins, especially in retinal pigment epithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells, and ganglion cells. In conclusion, CSP expression rapidly rises in the mammalian retina following exposure to hypothermia in a cell type-specific pattern. This observation may be at the basis of the molecular mechanism by which hypothermia exerts its therapeutic effects in the retina. Public Library of Science 2016-08-24 /pmc/articles/PMC4996528/ /pubmed/27556928 http://dx.doi.org/10.1371/journal.pone.0161458 Text en © 2016 Larrayoz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Larrayoz, Ignacio M.
Rey-Funes, Manuel
Contartese, Daniela S.
Rolón, Federico
Sarotto, Anibal
Dorfman, Veronica B.
Loidl, Cesar F.
Martínez, Alfredo
Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title_full Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title_fullStr Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title_full_unstemmed Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title_short Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
title_sort cold shock proteins are expressed in the retina following exposure to low temperatures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996528/
https://www.ncbi.nlm.nih.gov/pubmed/27556928
http://dx.doi.org/10.1371/journal.pone.0161458
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