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An integrative transcriptomic atlas of organogenesis in human embryos
Human organogenesis is when severe developmental abnormalities commonly originate. However, understanding this critical embryonic phase has relied upon inference from patient phenotypes and assumptions from in vitro stem cell models and non-human vertebrates. We report an integrated transcriptomic a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996651/ https://www.ncbi.nlm.nih.gov/pubmed/27557446 http://dx.doi.org/10.7554/eLife.15657 |
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author | Gerrard, Dave T Berry, Andrew A Jennings, Rachel E Piper Hanley, Karen Bobola, Nicoletta Hanley, Neil A |
author_facet | Gerrard, Dave T Berry, Andrew A Jennings, Rachel E Piper Hanley, Karen Bobola, Nicoletta Hanley, Neil A |
author_sort | Gerrard, Dave T |
collection | PubMed |
description | Human organogenesis is when severe developmental abnormalities commonly originate. However, understanding this critical embryonic phase has relied upon inference from patient phenotypes and assumptions from in vitro stem cell models and non-human vertebrates. We report an integrated transcriptomic atlas of human organogenesis. By lineage-guided principal components analysis, we uncover novel relatedness of particular developmental genes across different organs and tissues and identified unique transcriptional codes which correctly predicted the cause of many congenital disorders. By inference, our model pinpoints co-enriched genes as new causes of developmental disorders such as cleft palate and congenital heart disease. The data revealed more than 6000 novel transcripts, over 90% of which fulfil criteria as long non-coding RNAs correlated with the protein-coding genome over megabase distances. Taken together, we have uncovered cryptic transcriptional programs used by the human embryo and established a new resource for the molecular understanding of human organogenesis and its associated disorders. DOI: http://dx.doi.org/10.7554/eLife.15657.001 |
format | Online Article Text |
id | pubmed-4996651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-49966512016-08-29 An integrative transcriptomic atlas of organogenesis in human embryos Gerrard, Dave T Berry, Andrew A Jennings, Rachel E Piper Hanley, Karen Bobola, Nicoletta Hanley, Neil A eLife Developmental Biology and Stem Cells Human organogenesis is when severe developmental abnormalities commonly originate. However, understanding this critical embryonic phase has relied upon inference from patient phenotypes and assumptions from in vitro stem cell models and non-human vertebrates. We report an integrated transcriptomic atlas of human organogenesis. By lineage-guided principal components analysis, we uncover novel relatedness of particular developmental genes across different organs and tissues and identified unique transcriptional codes which correctly predicted the cause of many congenital disorders. By inference, our model pinpoints co-enriched genes as new causes of developmental disorders such as cleft palate and congenital heart disease. The data revealed more than 6000 novel transcripts, over 90% of which fulfil criteria as long non-coding RNAs correlated with the protein-coding genome over megabase distances. Taken together, we have uncovered cryptic transcriptional programs used by the human embryo and established a new resource for the molecular understanding of human organogenesis and its associated disorders. DOI: http://dx.doi.org/10.7554/eLife.15657.001 eLife Sciences Publications, Ltd 2016-08-24 /pmc/articles/PMC4996651/ /pubmed/27557446 http://dx.doi.org/10.7554/eLife.15657 Text en © 2016, Gerrard et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Gerrard, Dave T Berry, Andrew A Jennings, Rachel E Piper Hanley, Karen Bobola, Nicoletta Hanley, Neil A An integrative transcriptomic atlas of organogenesis in human embryos |
title | An integrative transcriptomic atlas of organogenesis in human embryos |
title_full | An integrative transcriptomic atlas of organogenesis in human embryos |
title_fullStr | An integrative transcriptomic atlas of organogenesis in human embryos |
title_full_unstemmed | An integrative transcriptomic atlas of organogenesis in human embryos |
title_short | An integrative transcriptomic atlas of organogenesis in human embryos |
title_sort | integrative transcriptomic atlas of organogenesis in human embryos |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996651/ https://www.ncbi.nlm.nih.gov/pubmed/27557446 http://dx.doi.org/10.7554/eLife.15657 |
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