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Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae

Incidence of natural light stress renders it important to enhance our understanding of the mechanisms by which plants protect themselves from harmful effects of UV-B irradiation, as this is critical for fitness of land plant species. Here we describe natural variation of a class of phenylacylated-fl...

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Autores principales: Tohge, Takayuki, Wendenburg, Regina, Ishihara, Hirofumi, Nakabayashi, Ryo, Watanabe, Mutsumi, Sulpice, Ronan, Hoefgen, Rainer, Takayama, Hiromitsu, Saito, Kazuki, Stitt, Mark, Fernie, Alisdair R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996938/
https://www.ncbi.nlm.nih.gov/pubmed/27545969
http://dx.doi.org/10.1038/ncomms12399
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author Tohge, Takayuki
Wendenburg, Regina
Ishihara, Hirofumi
Nakabayashi, Ryo
Watanabe, Mutsumi
Sulpice, Ronan
Hoefgen, Rainer
Takayama, Hiromitsu
Saito, Kazuki
Stitt, Mark
Fernie, Alisdair R.
author_facet Tohge, Takayuki
Wendenburg, Regina
Ishihara, Hirofumi
Nakabayashi, Ryo
Watanabe, Mutsumi
Sulpice, Ronan
Hoefgen, Rainer
Takayama, Hiromitsu
Saito, Kazuki
Stitt, Mark
Fernie, Alisdair R.
author_sort Tohge, Takayuki
collection PubMed
description Incidence of natural light stress renders it important to enhance our understanding of the mechanisms by which plants protect themselves from harmful effects of UV-B irradiation, as this is critical for fitness of land plant species. Here we describe natural variation of a class of phenylacylated-flavonols (saiginols), which accumulate to high levels in floral tissues of Arabidopsis. They were identified in a subset of accessions, especially those deriving from latitudes between 16° and 43° North. Investigation of introgression line populations using metabolic and transcript profiling, combined with genomic sequence analysis, allowed the identification of flavonol-phenylacyltransferase 2 (FPT2) that is responsible for the production of saiginols and conferring greater UV light tolerance in planta. Furthermore, analysis of polymorphism within the FPT duplicated region provides an evolutionary framework of the natural history of this locus in the Brassicaceae.
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spelling pubmed-49969382016-09-07 Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae Tohge, Takayuki Wendenburg, Regina Ishihara, Hirofumi Nakabayashi, Ryo Watanabe, Mutsumi Sulpice, Ronan Hoefgen, Rainer Takayama, Hiromitsu Saito, Kazuki Stitt, Mark Fernie, Alisdair R. Nat Commun Article Incidence of natural light stress renders it important to enhance our understanding of the mechanisms by which plants protect themselves from harmful effects of UV-B irradiation, as this is critical for fitness of land plant species. Here we describe natural variation of a class of phenylacylated-flavonols (saiginols), which accumulate to high levels in floral tissues of Arabidopsis. They were identified in a subset of accessions, especially those deriving from latitudes between 16° and 43° North. Investigation of introgression line populations using metabolic and transcript profiling, combined with genomic sequence analysis, allowed the identification of flavonol-phenylacyltransferase 2 (FPT2) that is responsible for the production of saiginols and conferring greater UV light tolerance in planta. Furthermore, analysis of polymorphism within the FPT duplicated region provides an evolutionary framework of the natural history of this locus in the Brassicaceae. Nature Publishing Group 2016-08-22 /pmc/articles/PMC4996938/ /pubmed/27545969 http://dx.doi.org/10.1038/ncomms12399 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tohge, Takayuki
Wendenburg, Regina
Ishihara, Hirofumi
Nakabayashi, Ryo
Watanabe, Mutsumi
Sulpice, Ronan
Hoefgen, Rainer
Takayama, Hiromitsu
Saito, Kazuki
Stitt, Mark
Fernie, Alisdair R.
Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title_full Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title_fullStr Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title_full_unstemmed Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title_short Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae
title_sort characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in brassicaceae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996938/
https://www.ncbi.nlm.nih.gov/pubmed/27545969
http://dx.doi.org/10.1038/ncomms12399
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