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Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution...

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Autores principales: Nogly, Przemyslaw, Panneels, Valerie, Nelson, Garrett, Gati, Cornelius, Kimura, Tetsunari, Milne, Christopher, Milathianaki, Despina, Kubo, Minoru, Wu, Wenting, Conrad, Chelsie, Coe, Jesse, Bean, Richard, Zhao, Yun, Båth, Petra, Dods, Robert, Harimoorthy, Rajiv, Beyerlein, Kenneth R., Rheinberger, Jan, James, Daniel, DePonte, Daniel, Li, Chufeng, Sala, Leonardo, Williams, Garth J., Hunter, Mark S., Koglin, Jason E., Berntsen, Peter, Nango, Eriko, Iwata, So, Chapman, Henry N., Fromme, Petra, Frank, Matthias, Abela, Rafael, Boutet, Sébastien, Barty, Anton, White, Thomas A., Weierstall, Uwe, Spence, John, Neutze, Richard, Schertler, Gebhard, Standfuss, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996941/
https://www.ncbi.nlm.nih.gov/pubmed/27545823
http://dx.doi.org/10.1038/ncomms12314
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author Nogly, Przemyslaw
Panneels, Valerie
Nelson, Garrett
Gati, Cornelius
Kimura, Tetsunari
Milne, Christopher
Milathianaki, Despina
Kubo, Minoru
Wu, Wenting
Conrad, Chelsie
Coe, Jesse
Bean, Richard
Zhao, Yun
Båth, Petra
Dods, Robert
Harimoorthy, Rajiv
Beyerlein, Kenneth R.
Rheinberger, Jan
James, Daniel
DePonte, Daniel
Li, Chufeng
Sala, Leonardo
Williams, Garth J.
Hunter, Mark S.
Koglin, Jason E.
Berntsen, Peter
Nango, Eriko
Iwata, So
Chapman, Henry N.
Fromme, Petra
Frank, Matthias
Abela, Rafael
Boutet, Sébastien
Barty, Anton
White, Thomas A.
Weierstall, Uwe
Spence, John
Neutze, Richard
Schertler, Gebhard
Standfuss, Jörg
author_facet Nogly, Przemyslaw
Panneels, Valerie
Nelson, Garrett
Gati, Cornelius
Kimura, Tetsunari
Milne, Christopher
Milathianaki, Despina
Kubo, Minoru
Wu, Wenting
Conrad, Chelsie
Coe, Jesse
Bean, Richard
Zhao, Yun
Båth, Petra
Dods, Robert
Harimoorthy, Rajiv
Beyerlein, Kenneth R.
Rheinberger, Jan
James, Daniel
DePonte, Daniel
Li, Chufeng
Sala, Leonardo
Williams, Garth J.
Hunter, Mark S.
Koglin, Jason E.
Berntsen, Peter
Nango, Eriko
Iwata, So
Chapman, Henry N.
Fromme, Petra
Frank, Matthias
Abela, Rafael
Boutet, Sébastien
Barty, Anton
White, Thomas A.
Weierstall, Uwe
Spence, John
Neutze, Richard
Schertler, Gebhard
Standfuss, Jörg
author_sort Nogly, Przemyslaw
collection PubMed
description Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
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spelling pubmed-49969412016-09-07 Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography Nogly, Przemyslaw Panneels, Valerie Nelson, Garrett Gati, Cornelius Kimura, Tetsunari Milne, Christopher Milathianaki, Despina Kubo, Minoru Wu, Wenting Conrad, Chelsie Coe, Jesse Bean, Richard Zhao, Yun Båth, Petra Dods, Robert Harimoorthy, Rajiv Beyerlein, Kenneth R. Rheinberger, Jan James, Daniel DePonte, Daniel Li, Chufeng Sala, Leonardo Williams, Garth J. Hunter, Mark S. Koglin, Jason E. Berntsen, Peter Nango, Eriko Iwata, So Chapman, Henry N. Fromme, Petra Frank, Matthias Abela, Rafael Boutet, Sébastien Barty, Anton White, Thomas A. Weierstall, Uwe Spence, John Neutze, Richard Schertler, Gebhard Standfuss, Jörg Nat Commun Article Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX. Nature Publishing Group 2016-08-22 /pmc/articles/PMC4996941/ /pubmed/27545823 http://dx.doi.org/10.1038/ncomms12314 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nogly, Przemyslaw
Panneels, Valerie
Nelson, Garrett
Gati, Cornelius
Kimura, Tetsunari
Milne, Christopher
Milathianaki, Despina
Kubo, Minoru
Wu, Wenting
Conrad, Chelsie
Coe, Jesse
Bean, Richard
Zhao, Yun
Båth, Petra
Dods, Robert
Harimoorthy, Rajiv
Beyerlein, Kenneth R.
Rheinberger, Jan
James, Daniel
DePonte, Daniel
Li, Chufeng
Sala, Leonardo
Williams, Garth J.
Hunter, Mark S.
Koglin, Jason E.
Berntsen, Peter
Nango, Eriko
Iwata, So
Chapman, Henry N.
Fromme, Petra
Frank, Matthias
Abela, Rafael
Boutet, Sébastien
Barty, Anton
White, Thomas A.
Weierstall, Uwe
Spence, John
Neutze, Richard
Schertler, Gebhard
Standfuss, Jörg
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title_full Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title_fullStr Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title_full_unstemmed Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title_short Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
title_sort lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996941/
https://www.ncbi.nlm.nih.gov/pubmed/27545823
http://dx.doi.org/10.1038/ncomms12314
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