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Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes

Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitr...

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Autor principal: Kim, Hye Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997377/
https://www.ncbi.nlm.nih.gov/pubmed/27483310
http://dx.doi.org/10.3390/nu8080464
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author Kim, Hye Kyung
author_facet Kim, Hye Kyung
author_sort Kim, Hye Kyung
collection PubMed
description Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitro cell free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and nitric oxide (NO). The effect of garlic extract on ROS formation, MMP-1 protein and mRNA expressions, cytokines such as interleukin (IL)-1β and IL-6, senescence associated-β-galactosidase (SA-β-gal) activity, and silent information regulator T1 (SIRT1) activity were determined in UVB-irradiated HaCaT cells. Garlic exhibited strong DPPH radical and NO scavenging activity in cell free system exhibiting IC(50) values of 2.50 mg/mL and 4.38 mg/mL, respectively. Garlic pretreatment attenuated the production of UVB-induced intracellular ROS. MMP-1 level, which has been known to be induced by ROS, was dramatically elevated by UVB irradiation, and UVB-induced MMP-1 mRNA and protein expressions were significantly reduced by garlic treatment (50 µg/mL) comparable to those of UV-unexposed control cells. UV-induced pro-inflammatory cytokine productions (IL-6 and IL-1β) were significantly inhibited by pretreatment with garlic in a dose-dependent manner. SA-β-gal activity, a classical biomarker of cellular senescence, and SIRT1 activity, which has attracted attention as an anti-aging factor in recent years, were ameliorated by garlic treatment in UV-irradiated HaCaT cells. The present study provides the first evidence of garlic inhibiting UVB-induced photoaging as a result of augmentation of cellular senescence in HaCaT human keratinocytes.
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spelling pubmed-49973772016-08-26 Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes Kim, Hye Kyung Nutrients Article Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitro cell free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and nitric oxide (NO). The effect of garlic extract on ROS formation, MMP-1 protein and mRNA expressions, cytokines such as interleukin (IL)-1β and IL-6, senescence associated-β-galactosidase (SA-β-gal) activity, and silent information regulator T1 (SIRT1) activity were determined in UVB-irradiated HaCaT cells. Garlic exhibited strong DPPH radical and NO scavenging activity in cell free system exhibiting IC(50) values of 2.50 mg/mL and 4.38 mg/mL, respectively. Garlic pretreatment attenuated the production of UVB-induced intracellular ROS. MMP-1 level, which has been known to be induced by ROS, was dramatically elevated by UVB irradiation, and UVB-induced MMP-1 mRNA and protein expressions were significantly reduced by garlic treatment (50 µg/mL) comparable to those of UV-unexposed control cells. UV-induced pro-inflammatory cytokine productions (IL-6 and IL-1β) were significantly inhibited by pretreatment with garlic in a dose-dependent manner. SA-β-gal activity, a classical biomarker of cellular senescence, and SIRT1 activity, which has attracted attention as an anti-aging factor in recent years, were ameliorated by garlic treatment in UV-irradiated HaCaT cells. The present study provides the first evidence of garlic inhibiting UVB-induced photoaging as a result of augmentation of cellular senescence in HaCaT human keratinocytes. MDPI 2016-07-29 /pmc/articles/PMC4997377/ /pubmed/27483310 http://dx.doi.org/10.3390/nu8080464 Text en © 2016 by the author; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hye Kyung
Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title_full Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title_fullStr Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title_full_unstemmed Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title_short Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
title_sort protective effect of garlic on cellular senescence in uvb-exposed hacat human keratinocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997377/
https://www.ncbi.nlm.nih.gov/pubmed/27483310
http://dx.doi.org/10.3390/nu8080464
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