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Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy

It is still unclear to which extent methyl-group intake during pregnancy can affect maternal global DNA (hydroxyl)methylation. Pregnancy methylation profiling and its link with methyl-group intake in a healthy population could enhance our understanding of the development of pregnancy related disorde...

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Autores principales: Pauwels, Sara, Duca, Radu Corneliu, Devlieger, Roland, Freson, Kathleen, Straetmans, Dany, Van Herck, Erik, Huybrechts, Inge, Koppen, Gurdun, Godderis, Lode
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997387/
https://www.ncbi.nlm.nih.gov/pubmed/27509522
http://dx.doi.org/10.3390/nu8080474
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author Pauwels, Sara
Duca, Radu Corneliu
Devlieger, Roland
Freson, Kathleen
Straetmans, Dany
Van Herck, Erik
Huybrechts, Inge
Koppen, Gurdun
Godderis, Lode
author_facet Pauwels, Sara
Duca, Radu Corneliu
Devlieger, Roland
Freson, Kathleen
Straetmans, Dany
Van Herck, Erik
Huybrechts, Inge
Koppen, Gurdun
Godderis, Lode
author_sort Pauwels, Sara
collection PubMed
description It is still unclear to which extent methyl-group intake during pregnancy can affect maternal global DNA (hydroxyl)methylation. Pregnancy methylation profiling and its link with methyl-group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring’s Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)methylation in blood using LC-MS/MS and dietary methyl-group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)methylation levels were highest pre-pregnancy and at weeks 18–22 of pregnancy. We observed a positive relation between folic acid and global DNA methylation (p = 0.04) and hydroxymethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)methylation percentage in weeks 11–13 and weeks 18–22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxymethylation. Women with a high intake of these three methyl groups in the second and third trimester showed higher hyrdoxymethylation/methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)methylation was found and women with higher methyl-group intake showed higher methylation in the third trimester, and not in earlier phases of pregnancy.
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spelling pubmed-49973872016-08-26 Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy Pauwels, Sara Duca, Radu Corneliu Devlieger, Roland Freson, Kathleen Straetmans, Dany Van Herck, Erik Huybrechts, Inge Koppen, Gurdun Godderis, Lode Nutrients Article It is still unclear to which extent methyl-group intake during pregnancy can affect maternal global DNA (hydroxyl)methylation. Pregnancy methylation profiling and its link with methyl-group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring’s Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)methylation in blood using LC-MS/MS and dietary methyl-group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)methylation levels were highest pre-pregnancy and at weeks 18–22 of pregnancy. We observed a positive relation between folic acid and global DNA methylation (p = 0.04) and hydroxymethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)methylation percentage in weeks 11–13 and weeks 18–22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxymethylation. Women with a high intake of these three methyl groups in the second and third trimester showed higher hyrdoxymethylation/methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)methylation was found and women with higher methyl-group intake showed higher methylation in the third trimester, and not in earlier phases of pregnancy. MDPI 2016-08-06 /pmc/articles/PMC4997387/ /pubmed/27509522 http://dx.doi.org/10.3390/nu8080474 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pauwels, Sara
Duca, Radu Corneliu
Devlieger, Roland
Freson, Kathleen
Straetmans, Dany
Van Herck, Erik
Huybrechts, Inge
Koppen, Gurdun
Godderis, Lode
Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title_full Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title_fullStr Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title_full_unstemmed Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title_short Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy
title_sort maternal methyl-group donor intake and global dna (hydroxy)methylation before and during pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997387/
https://www.ncbi.nlm.nih.gov/pubmed/27509522
http://dx.doi.org/10.3390/nu8080474
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