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Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells

To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm(2) for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined....

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Detalles Bibliográficos
Autores principales: Koyama, Shin, Narita, Eijiro, Shimizu, Yoko, Shiina, Takeo, Taki, Masao, Shinohara, Naoki, Miyakoshi, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997479/
https://www.ncbi.nlm.nih.gov/pubmed/27527204
http://dx.doi.org/10.3390/ijerph13080793
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author Koyama, Shin
Narita, Eijiro
Shimizu, Yoko
Shiina, Takeo
Taki, Masao
Shinohara, Naoki
Miyakoshi, Junji
author_facet Koyama, Shin
Narita, Eijiro
Shimizu, Yoko
Shiina, Takeo
Taki, Masao
Shinohara, Naoki
Miyakoshi, Junji
author_sort Koyama, Shin
collection PubMed
description To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm(2) for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye.
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spelling pubmed-49974792016-08-26 Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells Koyama, Shin Narita, Eijiro Shimizu, Yoko Shiina, Takeo Taki, Masao Shinohara, Naoki Miyakoshi, Junji Int J Environ Res Public Health Article To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm(2) for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye. MDPI 2016-08-05 2016-08 /pmc/articles/PMC4997479/ /pubmed/27527204 http://dx.doi.org/10.3390/ijerph13080793 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koyama, Shin
Narita, Eijiro
Shimizu, Yoko
Shiina, Takeo
Taki, Masao
Shinohara, Naoki
Miyakoshi, Junji
Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title_full Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title_fullStr Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title_full_unstemmed Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title_short Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells
title_sort twenty four-hour exposure to a 0.12 thz electromagnetic field does not affect the genotoxicity, morphological changes, or expression of heat shock protein in hce-t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997479/
https://www.ncbi.nlm.nih.gov/pubmed/27527204
http://dx.doi.org/10.3390/ijerph13080793
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