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Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease...

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Autores principales: García-Seco, Teresa, Pérez-Sancho, Marta, Salinas, Jesús, Navarro, Alejandro, Díez-Guerrier, Alberto, García, Nerea, Pozo, Pilar, Goyache, Joaquín, Domínguez, Lucas, Álvarez, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997790/
https://www.ncbi.nlm.nih.gov/pubmed/27610362
http://dx.doi.org/10.3389/fvets.2016.00067
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author García-Seco, Teresa
Pérez-Sancho, Marta
Salinas, Jesús
Navarro, Alejandro
Díez-Guerrier, Alberto
García, Nerea
Pozo, Pilar
Goyache, Joaquín
Domínguez, Lucas
Álvarez, Julio
author_facet García-Seco, Teresa
Pérez-Sancho, Marta
Salinas, Jesús
Navarro, Alejandro
Díez-Guerrier, Alberto
García, Nerea
Pozo, Pilar
Goyache, Joaquín
Domínguez, Lucas
Álvarez, Julio
author_sort García-Seco, Teresa
collection PubMed
description Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease but variable efficacy values have been described, possibly related with factors associated with the host, the vaccine, the parameter used for efficacy determination, and the challenge conditions. In this context, we evaluated the efficacy of an inactivated standard commercial vaccine and a 1/2 diluted dose in pregnant sheep challenged with C. abortus by examining multiple indicators of vaccine effect (including incidence of reproductive failures, bacterial excretion, and evolution of weight gain of viable lambs during the first month of life). Three groups of ewes [control non-vaccinated, C (n = 18); vaccinated with standard dose, SV (n = 16); and vaccinated with 1/2 dose, DV (n = 17)], were challenged approximately 90 days post-mating and tested using direct PCR (tissue samples and vaginal swabs) and ELISA (serum) until 31 days post-reproductive outcome. There were not significant differences in the proportions of reproductive failures or bacterial shedding after birth/abortion regardless the vaccination protocol. However, a beneficial effect of vaccination on offspring growth was detected in both vaccinated groups compared with the controls, with a mean increase in weight measured at 30 days of life of 1.5 and 2.5 kg (p = 0.056) and an increase in the geometric mean of the daily gain of 8.4 and 9.7% in lambs born from DV and SV ewes compared with controls, respectively. Our results demonstrate the effect of an inactivated vaccine in the development of the offspring of C. abortus-infected ewes at a standard and a diluted dose, an interesting finding given the difficulty in achieving sufficient antigen concentration in the production of enzootic abortion of ewes-commercial vaccines.
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spelling pubmed-49977902016-09-08 Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally García-Seco, Teresa Pérez-Sancho, Marta Salinas, Jesús Navarro, Alejandro Díez-Guerrier, Alberto García, Nerea Pozo, Pilar Goyache, Joaquín Domínguez, Lucas Álvarez, Julio Front Vet Sci Veterinary Science Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease but variable efficacy values have been described, possibly related with factors associated with the host, the vaccine, the parameter used for efficacy determination, and the challenge conditions. In this context, we evaluated the efficacy of an inactivated standard commercial vaccine and a 1/2 diluted dose in pregnant sheep challenged with C. abortus by examining multiple indicators of vaccine effect (including incidence of reproductive failures, bacterial excretion, and evolution of weight gain of viable lambs during the first month of life). Three groups of ewes [control non-vaccinated, C (n = 18); vaccinated with standard dose, SV (n = 16); and vaccinated with 1/2 dose, DV (n = 17)], were challenged approximately 90 days post-mating and tested using direct PCR (tissue samples and vaginal swabs) and ELISA (serum) until 31 days post-reproductive outcome. There were not significant differences in the proportions of reproductive failures or bacterial shedding after birth/abortion regardless the vaccination protocol. However, a beneficial effect of vaccination on offspring growth was detected in both vaccinated groups compared with the controls, with a mean increase in weight measured at 30 days of life of 1.5 and 2.5 kg (p = 0.056) and an increase in the geometric mean of the daily gain of 8.4 and 9.7% in lambs born from DV and SV ewes compared with controls, respectively. Our results demonstrate the effect of an inactivated vaccine in the development of the offspring of C. abortus-infected ewes at a standard and a diluted dose, an interesting finding given the difficulty in achieving sufficient antigen concentration in the production of enzootic abortion of ewes-commercial vaccines. Frontiers Media S.A. 2016-08-25 /pmc/articles/PMC4997790/ /pubmed/27610362 http://dx.doi.org/10.3389/fvets.2016.00067 Text en Copyright © 2016 García-Seco, Pérez-Sancho, Salinas, Navarro, Díez-Guerrier, García, Pozo, Goyache, Domínguez and Álvarez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
García-Seco, Teresa
Pérez-Sancho, Marta
Salinas, Jesús
Navarro, Alejandro
Díez-Guerrier, Alberto
García, Nerea
Pozo, Pilar
Goyache, Joaquín
Domínguez, Lucas
Álvarez, Julio
Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_full Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_fullStr Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_full_unstemmed Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_short Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_sort effect of preventive chlamydia abortus vaccination in offspring development in sheep challenged experimentally
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997790/
https://www.ncbi.nlm.nih.gov/pubmed/27610362
http://dx.doi.org/10.3389/fvets.2016.00067
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