Cargando…
Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography
PURPOSE: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology. METHODS: In the present study, OCT-A based...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997887/ https://www.ncbi.nlm.nih.gov/pubmed/27570710 http://dx.doi.org/10.1167/tvst.5.4.11 |
_version_ | 1782449847719165952 |
---|---|
author | Giannakaki-Zimmermann, Helena Kokona, Despina Wolf, Sebastian Ebneter, Andreas Zinkernagel, Martin S. |
author_facet | Giannakaki-Zimmermann, Helena Kokona, Despina Wolf, Sebastian Ebneter, Andreas Zinkernagel, Martin S. |
author_sort | Giannakaki-Zimmermann, Helena |
collection | PubMed |
description | PURPOSE: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology. METHODS: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities. RESULTS: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities. CONCLUSION: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus. TRANSLATIONAL RELEVANCE: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA. |
format | Online Article Text |
id | pubmed-4997887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49978872016-08-26 Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography Giannakaki-Zimmermann, Helena Kokona, Despina Wolf, Sebastian Ebneter, Andreas Zinkernagel, Martin S. Transl Vis Sci Technol Articles PURPOSE: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology. METHODS: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities. RESULTS: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities. CONCLUSION: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus. TRANSLATIONAL RELEVANCE: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA. The Association for Research in Vision and Ophthalmology 2016-08-18 /pmc/articles/PMC4997887/ /pubmed/27570710 http://dx.doi.org/10.1167/tvst.5.4.11 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Articles Giannakaki-Zimmermann, Helena Kokona, Despina Wolf, Sebastian Ebneter, Andreas Zinkernagel, Martin S. Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title | Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title_full | Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title_fullStr | Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title_full_unstemmed | Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title_short | Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography |
title_sort | optical coherence tomography angiography in mice: comparison with confocal scanning laser microscopy and fluorescein angiography |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997887/ https://www.ncbi.nlm.nih.gov/pubmed/27570710 http://dx.doi.org/10.1167/tvst.5.4.11 |
work_keys_str_mv | AT giannakakizimmermannhelena opticalcoherencetomographyangiographyinmicecomparisonwithconfocalscanninglasermicroscopyandfluoresceinangiography AT kokonadespina opticalcoherencetomographyangiographyinmicecomparisonwithconfocalscanninglasermicroscopyandfluoresceinangiography AT wolfsebastian opticalcoherencetomographyangiographyinmicecomparisonwithconfocalscanninglasermicroscopyandfluoresceinangiography AT ebneterandreas opticalcoherencetomographyangiographyinmicecomparisonwithconfocalscanninglasermicroscopyandfluoresceinangiography AT zinkernagelmartins opticalcoherencetomographyangiographyinmicecomparisonwithconfocalscanninglasermicroscopyandfluoresceinangiography |