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A new index to discriminate between iron deficiency anemia and thalassemia trait

BACKGROUND: The most common microcytic and hypochromic anemias are iron deficiency anemia and thalassemia trait. Several indices to discriminate iron deficiency anemia from thalassemia trait have been proposed as simple diagnostic tools. However, some of the best discriminative indices use parameter...

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Autores principales: Matos, Januária F., Dusse, Luci M.S., Borges, Karina B.G., de Castro, Ricardo L.V., Coura-Vital, Wendel, Carvalho, Maria das G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Hematologia e Hemoterapia 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997903/
https://www.ncbi.nlm.nih.gov/pubmed/27521859
http://dx.doi.org/10.1016/j.bjhh.2016.05.011
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author Matos, Januária F.
Dusse, Luci M.S.
Borges, Karina B.G.
de Castro, Ricardo L.V.
Coura-Vital, Wendel
Carvalho, Maria das G.
author_facet Matos, Januária F.
Dusse, Luci M.S.
Borges, Karina B.G.
de Castro, Ricardo L.V.
Coura-Vital, Wendel
Carvalho, Maria das G.
author_sort Matos, Januária F.
collection PubMed
description BACKGROUND: The most common microcytic and hypochromic anemias are iron deficiency anemia and thalassemia trait. Several indices to discriminate iron deficiency anemia from thalassemia trait have been proposed as simple diagnostic tools. However, some of the best discriminative indices use parameters in the formulas that are only measured in modern counters and are not always available in small laboratories. The development of an index with good diagnostic accuracy based only on parameters derived from the blood cell count obtained using simple counters would be useful in the clinical routine. Thus, the aim of this study was to develop and validate a discriminative index to differentiate iron deficiency anemia from thalassemia trait. METHODS: To develop and to validate the new formula, blood count data from 106 (thalassemia trait: 23 and iron deficiency: 83) and 185 patients (thalassemia trait: 30 and iron deficiency: 155) were used, respectively. Iron deficiency, β-thalassemia trait and α-thalassemia trait were confirmed by gold standard tests (low serum ferritin for iron deficiency anemia, HbA(2) > 3.5% for β-thalassemia trait and using molecular biology for the α-thalassemia trait). RESULTS: The sensitivity, specificity, efficiency, Youden's Index, area under receiver operating characteristic curve and Kappa coefficient of the new formula, called the Matos & Carvalho Index were 99.3%, 76.7%, 95.7%, 76.0, 0.95 and 0.83, respectively. CONCLUSION: The performance of this index was excellent with the advantage of being solely dependent on the mean corpuscular hemoglobin concentration and red blood cell count obtained from simple automatic counters and thus may be of great value in underdeveloped and developing countries.
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spelling pubmed-49979032016-09-02 A new index to discriminate between iron deficiency anemia and thalassemia trait Matos, Januária F. Dusse, Luci M.S. Borges, Karina B.G. de Castro, Ricardo L.V. Coura-Vital, Wendel Carvalho, Maria das G. Rev Bras Hematol Hemoter Original Article BACKGROUND: The most common microcytic and hypochromic anemias are iron deficiency anemia and thalassemia trait. Several indices to discriminate iron deficiency anemia from thalassemia trait have been proposed as simple diagnostic tools. However, some of the best discriminative indices use parameters in the formulas that are only measured in modern counters and are not always available in small laboratories. The development of an index with good diagnostic accuracy based only on parameters derived from the blood cell count obtained using simple counters would be useful in the clinical routine. Thus, the aim of this study was to develop and validate a discriminative index to differentiate iron deficiency anemia from thalassemia trait. METHODS: To develop and to validate the new formula, blood count data from 106 (thalassemia trait: 23 and iron deficiency: 83) and 185 patients (thalassemia trait: 30 and iron deficiency: 155) were used, respectively. Iron deficiency, β-thalassemia trait and α-thalassemia trait were confirmed by gold standard tests (low serum ferritin for iron deficiency anemia, HbA(2) > 3.5% for β-thalassemia trait and using molecular biology for the α-thalassemia trait). RESULTS: The sensitivity, specificity, efficiency, Youden's Index, area under receiver operating characteristic curve and Kappa coefficient of the new formula, called the Matos & Carvalho Index were 99.3%, 76.7%, 95.7%, 76.0, 0.95 and 0.83, respectively. CONCLUSION: The performance of this index was excellent with the advantage of being solely dependent on the mean corpuscular hemoglobin concentration and red blood cell count obtained from simple automatic counters and thus may be of great value in underdeveloped and developing countries. Sociedade Brasileira de Hematologia e Hemoterapia 2016 2016-06-22 /pmc/articles/PMC4997903/ /pubmed/27521859 http://dx.doi.org/10.1016/j.bjhh.2016.05.011 Text en © 2016 Associaç˜ao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Matos, Januária F.
Dusse, Luci M.S.
Borges, Karina B.G.
de Castro, Ricardo L.V.
Coura-Vital, Wendel
Carvalho, Maria das G.
A new index to discriminate between iron deficiency anemia and thalassemia trait
title A new index to discriminate between iron deficiency anemia and thalassemia trait
title_full A new index to discriminate between iron deficiency anemia and thalassemia trait
title_fullStr A new index to discriminate between iron deficiency anemia and thalassemia trait
title_full_unstemmed A new index to discriminate between iron deficiency anemia and thalassemia trait
title_short A new index to discriminate between iron deficiency anemia and thalassemia trait
title_sort new index to discriminate between iron deficiency anemia and thalassemia trait
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997903/
https://www.ncbi.nlm.nih.gov/pubmed/27521859
http://dx.doi.org/10.1016/j.bjhh.2016.05.011
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