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Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (P(f)), as well as the overall yield factor (PS/biomass) (Y(P/X)), 13 total cultivations distributed in 6 s...

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Autores principales: Baruque-Ramos, Julia, Juncioni de Arauz, Luciana, Fossa da Paz, Marcelo, Vicentin, Marcio Alberto, Hiss, Haroldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997934/
http://dx.doi.org/10.2174/2212711902666150901200052
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author Baruque-Ramos, Julia
Juncioni de Arauz, Luciana
Fossa da Paz, Marcelo
Vicentin, Marcio Alberto
Hiss, Haroldo
author_facet Baruque-Ramos, Julia
Juncioni de Arauz, Luciana
Fossa da Paz, Marcelo
Vicentin, Marcio Alberto
Hiss, Haroldo
author_sort Baruque-Ramos, Julia
collection PubMed
description Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (P(f)), as well as the overall yield factor (PS/biomass) (Y(P/X)), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35(o)C, 0.4 atm, 120 rpm, airflow rate of 5 L/min and K(L)a = 4.2 h(-1). The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O(2) controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10(th) hour; D) dissolved O(2) control at 10% saturation value; E) pH control at 7.4; F) dissolved O(2) limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of P(f) = 0.15 g/L and Y(P/X) = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.
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spelling pubmed-49979342016-08-31 Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C Baruque-Ramos, Julia Juncioni de Arauz, Luciana Fossa da Paz, Marcelo Vicentin, Marcio Alberto Hiss, Haroldo Curr Med Chem Article Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (P(f)), as well as the overall yield factor (PS/biomass) (Y(P/X)), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35(o)C, 0.4 atm, 120 rpm, airflow rate of 5 L/min and K(L)a = 4.2 h(-1). The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O(2) controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10(th) hour; D) dissolved O(2) control at 10% saturation value; E) pH control at 7.4; F) dissolved O(2) limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of P(f) = 0.15 g/L and Y(P/X) = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production. Bentham Science Publishers 2016-08 2016-08 /pmc/articles/PMC4997934/ http://dx.doi.org/10.2174/2212711902666150901200052 Text en © 2016 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Baruque-Ramos, Julia
Juncioni de Arauz, Luciana
Fossa da Paz, Marcelo
Vicentin, Marcio Alberto
Hiss, Haroldo
Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title_full Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title_fullStr Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title_full_unstemmed Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title_short Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
title_sort polysaccharide production in pilot scale bioreactor cultivations of neisseria meningitidis serogroup c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997934/
http://dx.doi.org/10.2174/2212711902666150901200052
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