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Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells
Despite the fact that the biological function of cluster of differentiation (CD)133 remains unclear, this glycoprotein is currently used in the identification and isolation of tumor-initiating cells from certain malignant tumors, including pancreatic cancer. In the present study, the involvement of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4998183/ https://www.ncbi.nlm.nih.gov/pubmed/27602113 http://dx.doi.org/10.3892/ol.2016.4888 |
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author | Sousa, Andreia Mota Rei, Margarida Freitas, Rita Ricardo, Sara Caffrey, Thomas David, Leonor Almeida, Raquel Hollingsworth, Michael Anthony Santos-Silva, Filipe |
author_facet | Sousa, Andreia Mota Rei, Margarida Freitas, Rita Ricardo, Sara Caffrey, Thomas David, Leonor Almeida, Raquel Hollingsworth, Michael Anthony Santos-Silva, Filipe |
author_sort | Sousa, Andreia Mota |
collection | PubMed |
description | Despite the fact that the biological function of cluster of differentiation (CD)133 remains unclear, this glycoprotein is currently used in the identification and isolation of tumor-initiating cells from certain malignant tumors, including pancreatic cancer. In the present study, the involvement of mucin 1 (MUC1) in the signaling pathways of a highly tumorigenic CD133+ cellular subpopulation sorted from the pancreatic cancer cell line HPAF-II was evaluated. The expression of MUC1-cytoplasmic domain (MUC1-CD) and oncogenic signaling transducers (epidermal growth factor receptor, protein kinase C delta, glycogen synthase kinase 3 beta and growth factor receptor-bound protein 2), as well as the association between MUC1 and β-catenin, were characterized in HPAF-II CD133+ and CD133low cell subpopulations and in tumor xenografts generated from these cells. Compared with HPAF CD133(low) cells, HPAF-II CD133+ cancer cells exhibited increased tumorigenic potential in immunocompromised mice, which was associated with overexpression of MUC1 and with the accordingly altered expression profile of MUC1-associated signaling partners. Additionally, MUC1-CD/β-catenin interactions were increased both in the HPAF-II CD133+ cell subpopulation and derived tumor xenografts compared with HPAF CD133(low) cells. These results suggest that, in comparison with HPAF CD133(low) cells, CD133+ cells exhibit higher expression of MUC1, which contributes to their tumorigenic phenotype through increased interaction between MUC1-CD and β-catenin, which in turn modulates oncogenic signaling cascades. |
format | Online Article Text |
id | pubmed-4998183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-49981832016-09-06 Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells Sousa, Andreia Mota Rei, Margarida Freitas, Rita Ricardo, Sara Caffrey, Thomas David, Leonor Almeida, Raquel Hollingsworth, Michael Anthony Santos-Silva, Filipe Oncol Lett Articles Despite the fact that the biological function of cluster of differentiation (CD)133 remains unclear, this glycoprotein is currently used in the identification and isolation of tumor-initiating cells from certain malignant tumors, including pancreatic cancer. In the present study, the involvement of mucin 1 (MUC1) in the signaling pathways of a highly tumorigenic CD133+ cellular subpopulation sorted from the pancreatic cancer cell line HPAF-II was evaluated. The expression of MUC1-cytoplasmic domain (MUC1-CD) and oncogenic signaling transducers (epidermal growth factor receptor, protein kinase C delta, glycogen synthase kinase 3 beta and growth factor receptor-bound protein 2), as well as the association between MUC1 and β-catenin, were characterized in HPAF-II CD133+ and CD133low cell subpopulations and in tumor xenografts generated from these cells. Compared with HPAF CD133(low) cells, HPAF-II CD133+ cancer cells exhibited increased tumorigenic potential in immunocompromised mice, which was associated with overexpression of MUC1 and with the accordingly altered expression profile of MUC1-associated signaling partners. Additionally, MUC1-CD/β-catenin interactions were increased both in the HPAF-II CD133+ cell subpopulation and derived tumor xenografts compared with HPAF CD133(low) cells. These results suggest that, in comparison with HPAF CD133(low) cells, CD133+ cells exhibit higher expression of MUC1, which contributes to their tumorigenic phenotype through increased interaction between MUC1-CD and β-catenin, which in turn modulates oncogenic signaling cascades. D.A. Spandidos 2016-09 2016-07-20 /pmc/articles/PMC4998183/ /pubmed/27602113 http://dx.doi.org/10.3892/ol.2016.4888 Text en Copyright: © Sousa et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Sousa, Andreia Mota Rei, Margarida Freitas, Rita Ricardo, Sara Caffrey, Thomas David, Leonor Almeida, Raquel Hollingsworth, Michael Anthony Santos-Silva, Filipe Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title | Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title_full | Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title_fullStr | Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title_full_unstemmed | Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title_short | Effect of MUC1/β-catenin interaction on the tumorigenic capacity of pancreatic CD133(+) cells |
title_sort | effect of muc1/β-catenin interaction on the tumorigenic capacity of pancreatic cd133(+) cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4998183/ https://www.ncbi.nlm.nih.gov/pubmed/27602113 http://dx.doi.org/10.3892/ol.2016.4888 |
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