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Molecular Identification of Biliary Isospora Belli: A Case Report

This report describes the novel sampling of bile from the biliary endoscopic intervention for the molecular identification of parasite infection. A 63-year-old Vietnamese man underwent travel health examination in our hospital. Physical examination showed that his height was 159 cm and weight was 41...

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Detalles Bibliográficos
Autores principales: Chiu, King-Wah, Chiou, Shue-Shian, Lu, Lung-Sheng, Wu, Cheng-Kun, Eng, Hock-Liew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4998921/
https://www.ncbi.nlm.nih.gov/pubmed/26962840
http://dx.doi.org/10.1097/MD.0000000000003071
Descripción
Sumario:This report describes the novel sampling of bile from the biliary endoscopic intervention for the molecular identification of parasite infection. A 63-year-old Vietnamese man underwent travel health examination in our hospital. Physical examination showed that his height was 159 cm and weight was 41 kg. He had a 15-year history of intermittent abdominal pain and frequent episodes of diarrhea. Laboratory tests revealed raised eosinophil count (23%, normal range [NR] 0–5), absolute eosinophil count (1899/μL, NR 50–350), and levels of serum immunoglobulin E (3770 IU/mL, NR < 100), aspartate transaminase (270 U/L, NR 0–37), alanine transaminase (210 U/L, NR 0–40), and total bilirubin (1.8 mg/dL, NR 0.2–1.4); however, the serum alkaline phosphatase level was normal (65 U/L, NR 28–94) and non-reactive result for serum human insufficiency virus antibody. Magnetic resonance cholangiopancreatography revealed diffuse dilatation of the biliary tree; the common hepatic and pancreatic duct diameters increased to 1.86 cm and 0.61 cm, respectively. Endoscopic retrograde cholangiopancreatography was performed and a 10-Fr model plastic biliary stent was inserted and flushed with 20 cc normal saline; thereafter, the bile was collected and sent for DNA sequencing. Isospora belli (IB) infection was identified by a polymerase chain reaction. Trimethoprim–sulfamethoxazole 800 mg q6h was administered for 1 month. Liver enzyme levels normalized and negative for concentration method of ova study. The patient was doing well and weighed 51 kg at the outpatient clinic visit 3 months later. This bile sampling with molecular identification has not been described in the literature. We believe that an acute IB infection through fecal-oral transmission may progress to chronic infection of the hepatobiliary system, leading to biliary obstruction and jaundice.