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Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes

Codon usage bias affects protein translation because tRNAs that recognize synonymous codons differ in their abundance. Although the current dogma states that tRNA expression is exclusively regulated by intrinsic control elements (A- and B-box sequences), we revealed, using a reporter that monitors t...

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Autores principales: Sagi, Dror, Rak, Roni, Gingold, Hila, Adir, Idan, Maayan, Gadi, Dahan, Orna, Broday, Limor, Pilpel, Yitzhak, Rechavi, Oded
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999229/
https://www.ncbi.nlm.nih.gov/pubmed/27560950
http://dx.doi.org/10.1371/journal.pgen.1006264
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author Sagi, Dror
Rak, Roni
Gingold, Hila
Adir, Idan
Maayan, Gadi
Dahan, Orna
Broday, Limor
Pilpel, Yitzhak
Rechavi, Oded
author_facet Sagi, Dror
Rak, Roni
Gingold, Hila
Adir, Idan
Maayan, Gadi
Dahan, Orna
Broday, Limor
Pilpel, Yitzhak
Rechavi, Oded
author_sort Sagi, Dror
collection PubMed
description Codon usage bias affects protein translation because tRNAs that recognize synonymous codons differ in their abundance. Although the current dogma states that tRNA expression is exclusively regulated by intrinsic control elements (A- and B-box sequences), we revealed, using a reporter that monitors the levels of individual tRNA genes in Caenorhabditis elegans, that eight tryptophan tRNA genes, 100% identical in sequence, are expressed in different tissues and change their expression dynamically. Furthermore, the expression levels of the sup-7 tRNA gene at day 6 were found to predict the animal’s lifespan. We discovered that the expression of tRNAs that reside within introns of protein-coding genes is affected by the host gene’s promoter. Pairing between specific Pol II genes and the tRNAs that are contained in their introns is most likely adaptive, since a genome-wide analysis revealed that the presence of specific intronic tRNAs within specific orthologous genes is conserved across Caenorhabditis species.
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spelling pubmed-49992292016-09-12 Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes Sagi, Dror Rak, Roni Gingold, Hila Adir, Idan Maayan, Gadi Dahan, Orna Broday, Limor Pilpel, Yitzhak Rechavi, Oded PLoS Genet Research Article Codon usage bias affects protein translation because tRNAs that recognize synonymous codons differ in their abundance. Although the current dogma states that tRNA expression is exclusively regulated by intrinsic control elements (A- and B-box sequences), we revealed, using a reporter that monitors the levels of individual tRNA genes in Caenorhabditis elegans, that eight tryptophan tRNA genes, 100% identical in sequence, are expressed in different tissues and change their expression dynamically. Furthermore, the expression levels of the sup-7 tRNA gene at day 6 were found to predict the animal’s lifespan. We discovered that the expression of tRNAs that reside within introns of protein-coding genes is affected by the host gene’s promoter. Pairing between specific Pol II genes and the tRNAs that are contained in their introns is most likely adaptive, since a genome-wide analysis revealed that the presence of specific intronic tRNAs within specific orthologous genes is conserved across Caenorhabditis species. Public Library of Science 2016-08-25 /pmc/articles/PMC4999229/ /pubmed/27560950 http://dx.doi.org/10.1371/journal.pgen.1006264 Text en © 2016 Sagi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sagi, Dror
Rak, Roni
Gingold, Hila
Adir, Idan
Maayan, Gadi
Dahan, Orna
Broday, Limor
Pilpel, Yitzhak
Rechavi, Oded
Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title_full Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title_fullStr Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title_full_unstemmed Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title_short Tissue- and Time-Specific Expression of Otherwise Identical tRNA Genes
title_sort tissue- and time-specific expression of otherwise identical trna genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999229/
https://www.ncbi.nlm.nih.gov/pubmed/27560950
http://dx.doi.org/10.1371/journal.pgen.1006264
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