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Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets
Age-related memory impairment (AMI) is a common feature and a debilitating phenotype of brain aging in many animals. However, the molecular mechanisms underlying AMI are still largely unknown. The cricket Gryllus bimaculatus is a useful experimental animal for studying age-related changes in learnin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999442/ https://www.ncbi.nlm.nih.gov/pubmed/27616985 http://dx.doi.org/10.3389/fnbeh.2016.00166 |
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author | Matsumoto, Yukihisa Matsumoto, Chihiro S. Takahashi, Toshihumi Mizunami, Makoto |
author_facet | Matsumoto, Yukihisa Matsumoto, Chihiro S. Takahashi, Toshihumi Mizunami, Makoto |
author_sort | Matsumoto, Yukihisa |
collection | PubMed |
description | Age-related memory impairment (AMI) is a common feature and a debilitating phenotype of brain aging in many animals. However, the molecular mechanisms underlying AMI are still largely unknown. The cricket Gryllus bimaculatus is a useful experimental animal for studying age-related changes in learning and memory capability; because the cricket has relatively short life-cycle and a high capability of olfactory learning and memory. Moreover, the molecular mechanisms underlying memory formation in crickets have been examined in detail. In the present study, we trained male crickets of different ages by multiple-trial olfactory conditioning to determine whether AMI occurs in crickets. Crickets 3 weeks after the final molt (3-week-old crickets) exhibited levels of retention similar to those of 1-week-old crickets at 30 min or 2 h after training; however they showed significantly decreased levels of 1-day retention, indicating AMI in long-term memory (LTM) but not in anesthesia-resistant memory (ARM) in olfactory learning of crickets. Furthermore, 3-week-old crickets injected with a nitric oxide (NO) donor, a cyclic GMP (cGMP) analog or a cyclic AMP (cAMP) analog into the hemolymph before conditioning exhibited a normal level of LTM, the same level as that in 1-week-old crickets. The rescue effect by NO donor or cGMP analog injection was absent when the crickets were injected after the conditioning. For the first time, an NO donor and a cGMP analog were found to antagonize the age-related impairment of LTM formation, suggesting that deterioration of NO synthase (NOS) or molecules upstream of NOS activation is involved in brain-aging processes. |
format | Online Article Text |
id | pubmed-4999442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49994422016-09-09 Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets Matsumoto, Yukihisa Matsumoto, Chihiro S. Takahashi, Toshihumi Mizunami, Makoto Front Behav Neurosci Neuroscience Age-related memory impairment (AMI) is a common feature and a debilitating phenotype of brain aging in many animals. However, the molecular mechanisms underlying AMI are still largely unknown. The cricket Gryllus bimaculatus is a useful experimental animal for studying age-related changes in learning and memory capability; because the cricket has relatively short life-cycle and a high capability of olfactory learning and memory. Moreover, the molecular mechanisms underlying memory formation in crickets have been examined in detail. In the present study, we trained male crickets of different ages by multiple-trial olfactory conditioning to determine whether AMI occurs in crickets. Crickets 3 weeks after the final molt (3-week-old crickets) exhibited levels of retention similar to those of 1-week-old crickets at 30 min or 2 h after training; however they showed significantly decreased levels of 1-day retention, indicating AMI in long-term memory (LTM) but not in anesthesia-resistant memory (ARM) in olfactory learning of crickets. Furthermore, 3-week-old crickets injected with a nitric oxide (NO) donor, a cyclic GMP (cGMP) analog or a cyclic AMP (cAMP) analog into the hemolymph before conditioning exhibited a normal level of LTM, the same level as that in 1-week-old crickets. The rescue effect by NO donor or cGMP analog injection was absent when the crickets were injected after the conditioning. For the first time, an NO donor and a cGMP analog were found to antagonize the age-related impairment of LTM formation, suggesting that deterioration of NO synthase (NOS) or molecules upstream of NOS activation is involved in brain-aging processes. Frontiers Media S.A. 2016-08-26 /pmc/articles/PMC4999442/ /pubmed/27616985 http://dx.doi.org/10.3389/fnbeh.2016.00166 Text en Copyright © 2016 Matsumoto, Matsumoto, Takahashi and Mizunami. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Matsumoto, Yukihisa Matsumoto, Chihiro S. Takahashi, Toshihumi Mizunami, Makoto Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title | Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title_full | Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title_fullStr | Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title_full_unstemmed | Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title_short | Activation of NO-cGMP Signaling Rescues Age-Related Memory Impairment in Crickets |
title_sort | activation of no-cgmp signaling rescues age-related memory impairment in crickets |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999442/ https://www.ncbi.nlm.nih.gov/pubmed/27616985 http://dx.doi.org/10.3389/fnbeh.2016.00166 |
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