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Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria

In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to he...

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Autores principales: Olofsson, Tobias C., Butler, Éile, Lindholm, Christina, Nilson, Bo, Michanek, Per, Vásquez, Alejandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999459/
https://www.ncbi.nlm.nih.gov/pubmed/27324340
http://dx.doi.org/10.1007/s00284-016-1080-2
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author Olofsson, Tobias C.
Butler, Éile
Lindholm, Christina
Nilson, Bo
Michanek, Per
Vásquez, Alejandra
author_facet Olofsson, Tobias C.
Butler, Éile
Lindholm, Christina
Nilson, Bo
Michanek, Per
Vásquez, Alejandra
author_sort Olofsson, Tobias C.
collection PubMed
description In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens. The aim of this pilot study was to investigate the effects on the healing of hard-to-heal equine wounds after treatment with these LAB symbionts viable in a heather honey formulation. For this, we included ten horses with wound duration of >1 year, investigated the wound microbiota, and treated wounds with the novel honeybee LAB formulation. We identified the microbiota using MALDI-TOF mass spectrometry and DNA sequencing. In addition, the antimicrobial properties of the honeybee LAB formulation were tested against all wound isolates in vitro. Our results indicate a diverse wound microbiota including fifty-three bacterial species that showed 90 % colonization by at least one species of Staphylococcus. Treatment with the formulation promoted wound healing in all cases already after the first application and the wounds were either completely healed (n = 3) in less than 20 days or healing was in progress. Furthermore, the honeybee LAB formulation inhibited all pathogens when tested in vitro. Consequently, this new treatment option presents as a powerful candidate for the topical treatment of hard-to-heal wounds in horses.
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spelling pubmed-49994592016-09-12 Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria Olofsson, Tobias C. Butler, Éile Lindholm, Christina Nilson, Bo Michanek, Per Vásquez, Alejandra Curr Microbiol Article In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens. The aim of this pilot study was to investigate the effects on the healing of hard-to-heal equine wounds after treatment with these LAB symbionts viable in a heather honey formulation. For this, we included ten horses with wound duration of >1 year, investigated the wound microbiota, and treated wounds with the novel honeybee LAB formulation. We identified the microbiota using MALDI-TOF mass spectrometry and DNA sequencing. In addition, the antimicrobial properties of the honeybee LAB formulation were tested against all wound isolates in vitro. Our results indicate a diverse wound microbiota including fifty-three bacterial species that showed 90 % colonization by at least one species of Staphylococcus. Treatment with the formulation promoted wound healing in all cases already after the first application and the wounds were either completely healed (n = 3) in less than 20 days or healing was in progress. Furthermore, the honeybee LAB formulation inhibited all pathogens when tested in vitro. Consequently, this new treatment option presents as a powerful candidate for the topical treatment of hard-to-heal wounds in horses. Springer US 2016-06-21 2016 /pmc/articles/PMC4999459/ /pubmed/27324340 http://dx.doi.org/10.1007/s00284-016-1080-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Olofsson, Tobias C.
Butler, Éile
Lindholm, Christina
Nilson, Bo
Michanek, Per
Vásquez, Alejandra
Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title_full Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title_fullStr Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title_full_unstemmed Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title_short Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
title_sort fighting off wound pathogens in horses with honeybee lactic acid bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999459/
https://www.ncbi.nlm.nih.gov/pubmed/27324340
http://dx.doi.org/10.1007/s00284-016-1080-2
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