Cargando…
Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells
This study is aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) in colorectal cancer and its potential biological function. Colorectal adenoma is the precancerous lesions of colorectal cancer, so in this study, we used colorectal adenoma as negative control. The global...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999473/ https://www.ncbi.nlm.nih.gov/pubmed/26820130 http://dx.doi.org/10.1007/s13277-015-4350-x |
_version_ | 1782450126702247936 |
---|---|
author | Niu, Hui Hu, Zhaoyang Liu, Hui Hu, Guoliang Yang, Bo Wu, Shixiu Li, Fang |
author_facet | Niu, Hui Hu, Zhaoyang Liu, Hui Hu, Guoliang Yang, Bo Wu, Shixiu Li, Fang |
author_sort | Niu, Hui |
collection | PubMed |
description | This study is aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) in colorectal cancer and its potential biological function. Colorectal adenoma is the precancerous lesions of colorectal cancer, so in this study, we used colorectal adenoma as negative control. The global lncRNA expression profile in colorectal cancer and adenoma was evaluated by bioinformatics. The biological functions and potential mechanism of AK027294 were investigated in HCT116, HCT8, and SW480 colorectal cancer cells. A total of 135 lncRNAs were found to be differentially expressed in colorectal cancer and adenoma tissues. Among them, 71 lncRNAs were up-regulated and 64 lncRNAs were down-regulated. Especially, AK027294 was found to be highly expressed in colorectal cancer tissues compared with colorectal adenoma tissues (fold change is 184.5). Our results indicated that AK027294 down-regulation significantly inhibited colorectal cancer cells proliferation and migration, but promoted cell apoptosis (P < 0.05). The potential mechanism of AK027294 might be associated with the regulation of caspase-3, caspase-8, Bcl-2, MMP12, MMP9, and TWIST. The lncRNA expression profile in colorectal cancer suggests lncRNAs may play important roles in the occurrence and progression of colorectal cancer. AK027294 is highly expressed in colorectal cancer and closely correlates with colorectal cells proliferation, migration, and apoptosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13277-015-4350-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4999473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-49994732016-09-12 Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells Niu, Hui Hu, Zhaoyang Liu, Hui Hu, Guoliang Yang, Bo Wu, Shixiu Li, Fang Tumour Biol Original Article This study is aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) in colorectal cancer and its potential biological function. Colorectal adenoma is the precancerous lesions of colorectal cancer, so in this study, we used colorectal adenoma as negative control. The global lncRNA expression profile in colorectal cancer and adenoma was evaluated by bioinformatics. The biological functions and potential mechanism of AK027294 were investigated in HCT116, HCT8, and SW480 colorectal cancer cells. A total of 135 lncRNAs were found to be differentially expressed in colorectal cancer and adenoma tissues. Among them, 71 lncRNAs were up-regulated and 64 lncRNAs were down-regulated. Especially, AK027294 was found to be highly expressed in colorectal cancer tissues compared with colorectal adenoma tissues (fold change is 184.5). Our results indicated that AK027294 down-regulation significantly inhibited colorectal cancer cells proliferation and migration, but promoted cell apoptosis (P < 0.05). The potential mechanism of AK027294 might be associated with the regulation of caspase-3, caspase-8, Bcl-2, MMP12, MMP9, and TWIST. The lncRNA expression profile in colorectal cancer suggests lncRNAs may play important roles in the occurrence and progression of colorectal cancer. AK027294 is highly expressed in colorectal cancer and closely correlates with colorectal cells proliferation, migration, and apoptosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13277-015-4350-x) contains supplementary material, which is available to authorized users. Springer Netherlands 2016-01-28 /pmc/articles/PMC4999473/ /pubmed/26820130 http://dx.doi.org/10.1007/s13277-015-4350-x Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Niu, Hui Hu, Zhaoyang Liu, Hui Hu, Guoliang Yang, Bo Wu, Shixiu Li, Fang Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title | Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title_full | Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title_fullStr | Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title_full_unstemmed | Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title_short | Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
title_sort | long non-coding rna ak027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999473/ https://www.ncbi.nlm.nih.gov/pubmed/26820130 http://dx.doi.org/10.1007/s13277-015-4350-x |
work_keys_str_mv | AT niuhui longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT huzhaoyang longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT liuhui longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT huguoliang longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT yangbo longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT wushixiu longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells AT lifang longnoncodingrnaak027294involvesintheprocessofproliferationmigrationandapoptosisofcolorectalcancercells |