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Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice
Most of the deaths that occurred during two large outbreaks of Streptococcus suis infections in 1998 and 2005 in China were caused by streptococcal toxic shock syndrome (STSS), which is characterized by increased vascular permeability. Heparin-binding protein (HBP) is thought to mediate the vascular...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999480/ https://www.ncbi.nlm.nih.gov/pubmed/27617009 http://dx.doi.org/10.3389/fmicb.2016.01338 |
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author | Chen, Shaolong Xie, Wenlong Wu, Kai Li, Ping Ren, Zhiqiang Li, Lin Yuan, Yuan Zhang, Chunmao Zheng, Yuling Lv, Qingyu Jiang, Hua Jiang, Yongqiang |
author_facet | Chen, Shaolong Xie, Wenlong Wu, Kai Li, Ping Ren, Zhiqiang Li, Lin Yuan, Yuan Zhang, Chunmao Zheng, Yuling Lv, Qingyu Jiang, Hua Jiang, Yongqiang |
author_sort | Chen, Shaolong |
collection | PubMed |
description | Most of the deaths that occurred during two large outbreaks of Streptococcus suis infections in 1998 and 2005 in China were caused by streptococcal toxic shock syndrome (STSS), which is characterized by increased vascular permeability. Heparin-binding protein (HBP) is thought to mediate the vascular leakage. The purpose of this study was to investigate the detailed mechanism underlying the release of HBP and the vascular leakage induced by S. suis. Significantly higher serum levels of HBP were detected in Chinese patients with STSS than in patients with meningitis or healthy controls. Suilysin (SLY) is an exotoxin secreted by the highly virulent strain 05ZYH33, and it stimulated the release of HBP from the polymorphonuclear neutrophils and mediated vascular leakage in mice. The release of HBP induced by SLY was caused by a calcium influx-dependent degranulation. Analyses using a pharmacological approach revealed that the release of HBP induced by SLY was related to Toll-like receptor 4, p38 mitogen-activated protein kinase, and the 1-phosphatidylinositol 3-kinase pathway. It was also dependent on a G protein-coupled seven-membrane spanning receptor. The results of this study provide new insights into the vascular leakage in STSS associated with non-Group A streptococci, which could lead to the discovery of potential therapeutic targets for STSS associated with S. suis. |
format | Online Article Text |
id | pubmed-4999480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49994802016-09-09 Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice Chen, Shaolong Xie, Wenlong Wu, Kai Li, Ping Ren, Zhiqiang Li, Lin Yuan, Yuan Zhang, Chunmao Zheng, Yuling Lv, Qingyu Jiang, Hua Jiang, Yongqiang Front Microbiol Microbiology Most of the deaths that occurred during two large outbreaks of Streptococcus suis infections in 1998 and 2005 in China were caused by streptococcal toxic shock syndrome (STSS), which is characterized by increased vascular permeability. Heparin-binding protein (HBP) is thought to mediate the vascular leakage. The purpose of this study was to investigate the detailed mechanism underlying the release of HBP and the vascular leakage induced by S. suis. Significantly higher serum levels of HBP were detected in Chinese patients with STSS than in patients with meningitis or healthy controls. Suilysin (SLY) is an exotoxin secreted by the highly virulent strain 05ZYH33, and it stimulated the release of HBP from the polymorphonuclear neutrophils and mediated vascular leakage in mice. The release of HBP induced by SLY was caused by a calcium influx-dependent degranulation. Analyses using a pharmacological approach revealed that the release of HBP induced by SLY was related to Toll-like receptor 4, p38 mitogen-activated protein kinase, and the 1-phosphatidylinositol 3-kinase pathway. It was also dependent on a G protein-coupled seven-membrane spanning receptor. The results of this study provide new insights into the vascular leakage in STSS associated with non-Group A streptococci, which could lead to the discovery of potential therapeutic targets for STSS associated with S. suis. Frontiers Media S.A. 2016-08-26 /pmc/articles/PMC4999480/ /pubmed/27617009 http://dx.doi.org/10.3389/fmicb.2016.01338 Text en Copyright © 2016 Chen, Xie, Wu, Li, Ren, Li, Yuan, Zhang, Zheng, Lv, Jiang and Jiang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Shaolong Xie, Wenlong Wu, Kai Li, Ping Ren, Zhiqiang Li, Lin Yuan, Yuan Zhang, Chunmao Zheng, Yuling Lv, Qingyu Jiang, Hua Jiang, Yongqiang Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title | Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title_full | Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title_fullStr | Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title_full_unstemmed | Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title_short | Suilysin Stimulates the Release of Heparin Binding Protein from Neutrophils and Increases Vascular Permeability in Mice |
title_sort | suilysin stimulates the release of heparin binding protein from neutrophils and increases vascular permeability in mice |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999480/ https://www.ncbi.nlm.nih.gov/pubmed/27617009 http://dx.doi.org/10.3389/fmicb.2016.01338 |
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