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The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression

The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing...

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Autores principales: Fukumura, Kazuhiro, Wakabayashi, Shunichi, Kataoka, Naoyuki, Sakamoto, Hiroshi, Suzuki, Yutaka, Nakai, Kenta, Mayeda, Akila, Inoue, Kunio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000587/
https://www.ncbi.nlm.nih.gov/pubmed/27490541
http://dx.doi.org/10.3390/ijms17081153
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author Fukumura, Kazuhiro
Wakabayashi, Shunichi
Kataoka, Naoyuki
Sakamoto, Hiroshi
Suzuki, Yutaka
Nakai, Kenta
Mayeda, Akila
Inoue, Kunio
author_facet Fukumura, Kazuhiro
Wakabayashi, Shunichi
Kataoka, Naoyuki
Sakamoto, Hiroshi
Suzuki, Yutaka
Nakai, Kenta
Mayeda, Akila
Inoue, Kunio
author_sort Fukumura, Kazuhiro
collection PubMed
description The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
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spelling pubmed-50005872016-09-01 The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression Fukumura, Kazuhiro Wakabayashi, Shunichi Kataoka, Naoyuki Sakamoto, Hiroshi Suzuki, Yutaka Nakai, Kenta Mayeda, Akila Inoue, Kunio Int J Mol Sci Article The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes. MDPI 2016-08-02 /pmc/articles/PMC5000587/ /pubmed/27490541 http://dx.doi.org/10.3390/ijms17081153 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fukumura, Kazuhiro
Wakabayashi, Shunichi
Kataoka, Naoyuki
Sakamoto, Hiroshi
Suzuki, Yutaka
Nakai, Kenta
Mayeda, Akila
Inoue, Kunio
The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title_full The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title_fullStr The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title_full_unstemmed The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title_short The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
title_sort exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000587/
https://www.ncbi.nlm.nih.gov/pubmed/27490541
http://dx.doi.org/10.3390/ijms17081153
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