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Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus
In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F(1) generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000696/ https://www.ncbi.nlm.nih.gov/pubmed/27556458 http://dx.doi.org/10.3390/ijms17081299 |
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author | Zhou, He Ma, Tian-Yu Zhang, Rui Xu, Qi-Zheng Shen, Fu Qin, Yan-Jie Xu, Wen Wang, Yuan Li, Ya-Juan |
author_facet | Zhou, He Ma, Tian-Yu Zhang, Rui Xu, Qi-Zheng Shen, Fu Qin, Yan-Jie Xu, Wen Wang, Yuan Li, Ya-Juan |
author_sort | Zhou, He |
collection | PubMed |
description | In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F(1) generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established by our laboratory to explore methylation levels and pattern diversification features at the whole genome level of the polyploidy loach. The results showed that the total methylation and full methylation rates decreased on increased ploidy individuals; moreover, the hemimethylation rate showed no consistent pattern. Compared with diploid loach, the methylation patterns of tetraploid sites changed 68.17%, and the methylation patterns of triploid sites changed 73.05%. The proportion of hypermethylation genes is significantly higher than the proportion of demethylation genes. The methylation level of reciprocal cross F(1) generation is lower than the male diploid and higher than the female tetraploid. The hemimethylation and total methylation rate of the cross hybrid F(1) generation is significantly higher than the orthogonal F(1) generation (p < 0.01). After readjusting, the methylation pattern of genome DNA of reciprocal hybrids changed 69.59% and 72.83%, respectively. |
format | Online Article Text |
id | pubmed-5000696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50006962016-09-01 Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus Zhou, He Ma, Tian-Yu Zhang, Rui Xu, Qi-Zheng Shen, Fu Qin, Yan-Jie Xu, Wen Wang, Yuan Li, Ya-Juan Int J Mol Sci Article In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F(1) generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established by our laboratory to explore methylation levels and pattern diversification features at the whole genome level of the polyploidy loach. The results showed that the total methylation and full methylation rates decreased on increased ploidy individuals; moreover, the hemimethylation rate showed no consistent pattern. Compared with diploid loach, the methylation patterns of tetraploid sites changed 68.17%, and the methylation patterns of triploid sites changed 73.05%. The proportion of hypermethylation genes is significantly higher than the proportion of demethylation genes. The methylation level of reciprocal cross F(1) generation is lower than the male diploid and higher than the female tetraploid. The hemimethylation and total methylation rate of the cross hybrid F(1) generation is significantly higher than the orthogonal F(1) generation (p < 0.01). After readjusting, the methylation pattern of genome DNA of reciprocal hybrids changed 69.59% and 72.83%, respectively. MDPI 2016-08-22 /pmc/articles/PMC5000696/ /pubmed/27556458 http://dx.doi.org/10.3390/ijms17081299 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhou, He Ma, Tian-Yu Zhang, Rui Xu, Qi-Zheng Shen, Fu Qin, Yan-Jie Xu, Wen Wang, Yuan Li, Ya-Juan Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus |
title | Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus
anguillicaudatus |
title_full | Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus
anguillicaudatus |
title_fullStr | Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus
anguillicaudatus |
title_full_unstemmed | Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus
anguillicaudatus |
title_short | Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus
anguillicaudatus |
title_sort | analysis of different ploidy and parent–offspring genomic dna methylation in the loach misgurnus
anguillicaudatus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000696/ https://www.ncbi.nlm.nih.gov/pubmed/27556458 http://dx.doi.org/10.3390/ijms17081299 |
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