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Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats

Bile duct ligation (BDL)-treated rats display cholestasis and liver damages. The potential protective activity of melatonin in young BDL rats in terms of apoptosis, mitochondrial function, and endoplasmic reticulum (ER) homeostasis has not yet been evaluated. Three groups of young male Sprague-Dawle...

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Autores principales: Sheen, Jiunn-Ming, Chen, Yu-Chieh, Hsu, Mei-Hsin, Tain, You-Lin, Huang, Ying-Hsien, Tiao, Mao-Meng, Li, Shih-Wen, Huang, Li-Tung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000760/
https://www.ncbi.nlm.nih.gov/pubmed/27556445
http://dx.doi.org/10.3390/ijms17081365
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author Sheen, Jiunn-Ming
Chen, Yu-Chieh
Hsu, Mei-Hsin
Tain, You-Lin
Huang, Ying-Hsien
Tiao, Mao-Meng
Li, Shih-Wen
Huang, Li-Tung
author_facet Sheen, Jiunn-Ming
Chen, Yu-Chieh
Hsu, Mei-Hsin
Tain, You-Lin
Huang, Ying-Hsien
Tiao, Mao-Meng
Li, Shih-Wen
Huang, Li-Tung
author_sort Sheen, Jiunn-Ming
collection PubMed
description Bile duct ligation (BDL)-treated rats display cholestasis and liver damages. The potential protective activity of melatonin in young BDL rats in terms of apoptosis, mitochondrial function, and endoplasmic reticulum (ER) homeostasis has not yet been evaluated. Three groups of young male Sprague-Dawley rats were used: one group received laparotomy (Sham), a second group received BDL for two weeks (BDL), and a third group received BDL and intraperitoneal melatonin (100 mg/day) for two weeks (BDL + M). BDL group rats showed liver apoptosis, increased pro-inflamamtory mediators, caspases alterations, anti-apoptotic factors changes, and dysfunction of ER homeostasis. Melatonin effectively reversed apoptosis, mainly through intrinsic pathway and reversed ER stress. In addition, in vitro study showed melatonin exerted its effect mainly through the melatonin 2 receptor (MT2) in HepG2 cells. In conclusion, BDL in young rats caused liver apoptosis. Melatonin rescued the apoptotic changes via the intrinsic pathway, and possibly through the MT2 receptor. Melatonin also reversed ER stress induced by BDL.
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spelling pubmed-50007602016-09-01 Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats Sheen, Jiunn-Ming Chen, Yu-Chieh Hsu, Mei-Hsin Tain, You-Lin Huang, Ying-Hsien Tiao, Mao-Meng Li, Shih-Wen Huang, Li-Tung Int J Mol Sci Article Bile duct ligation (BDL)-treated rats display cholestasis and liver damages. The potential protective activity of melatonin in young BDL rats in terms of apoptosis, mitochondrial function, and endoplasmic reticulum (ER) homeostasis has not yet been evaluated. Three groups of young male Sprague-Dawley rats were used: one group received laparotomy (Sham), a second group received BDL for two weeks (BDL), and a third group received BDL and intraperitoneal melatonin (100 mg/day) for two weeks (BDL + M). BDL group rats showed liver apoptosis, increased pro-inflamamtory mediators, caspases alterations, anti-apoptotic factors changes, and dysfunction of ER homeostasis. Melatonin effectively reversed apoptosis, mainly through intrinsic pathway and reversed ER stress. In addition, in vitro study showed melatonin exerted its effect mainly through the melatonin 2 receptor (MT2) in HepG2 cells. In conclusion, BDL in young rats caused liver apoptosis. Melatonin rescued the apoptotic changes via the intrinsic pathway, and possibly through the MT2 receptor. Melatonin also reversed ER stress induced by BDL. MDPI 2016-08-20 /pmc/articles/PMC5000760/ /pubmed/27556445 http://dx.doi.org/10.3390/ijms17081365 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sheen, Jiunn-Ming
Chen, Yu-Chieh
Hsu, Mei-Hsin
Tain, You-Lin
Huang, Ying-Hsien
Tiao, Mao-Meng
Li, Shih-Wen
Huang, Li-Tung
Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title_full Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title_fullStr Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title_full_unstemmed Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title_short Melatonin Alleviates Liver Apoptosis in Bile Duct Ligation Young Rats
title_sort melatonin alleviates liver apoptosis in bile duct ligation young rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000760/
https://www.ncbi.nlm.nih.gov/pubmed/27556445
http://dx.doi.org/10.3390/ijms17081365
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