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Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model

Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone me...

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Autores principales: Mercatali, Laura, La Manna, Federico, Groenewoud, Arwin, Casadei, Roberto, Recine, Federica, Miserocchi, Giacomo, Pieri, Federica, Liverani, Chiara, Bongiovanni, Alberto, Spadazzi, Chiara, de Vita, Alessandro, van der Pluijm, Gabri, Giorgini, Andrea, Biagini, Roberto, Amadori, Dino, Ibrahim, Toni, Snaar-Jagalska, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000770/
https://www.ncbi.nlm.nih.gov/pubmed/27556456
http://dx.doi.org/10.3390/ijms17081375
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author Mercatali, Laura
La Manna, Federico
Groenewoud, Arwin
Casadei, Roberto
Recine, Federica
Miserocchi, Giacomo
Pieri, Federica
Liverani, Chiara
Bongiovanni, Alberto
Spadazzi, Chiara
de Vita, Alessandro
van der Pluijm, Gabri
Giorgini, Andrea
Biagini, Roberto
Amadori, Dino
Ibrahim, Toni
Snaar-Jagalska, Ewa
author_facet Mercatali, Laura
La Manna, Federico
Groenewoud, Arwin
Casadei, Roberto
Recine, Federica
Miserocchi, Giacomo
Pieri, Federica
Liverani, Chiara
Bongiovanni, Alberto
Spadazzi, Chiara
de Vita, Alessandro
van der Pluijm, Gabri
Giorgini, Andrea
Biagini, Roberto
Amadori, Dino
Ibrahim, Toni
Snaar-Jagalska, Ewa
author_sort Mercatali, Laura
collection PubMed
description Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model.
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spelling pubmed-50007702016-09-01 Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model Mercatali, Laura La Manna, Federico Groenewoud, Arwin Casadei, Roberto Recine, Federica Miserocchi, Giacomo Pieri, Federica Liverani, Chiara Bongiovanni, Alberto Spadazzi, Chiara de Vita, Alessandro van der Pluijm, Gabri Giorgini, Andrea Biagini, Roberto Amadori, Dino Ibrahim, Toni Snaar-Jagalska, Ewa Int J Mol Sci Article Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model. MDPI 2016-08-22 /pmc/articles/PMC5000770/ /pubmed/27556456 http://dx.doi.org/10.3390/ijms17081375 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mercatali, Laura
La Manna, Federico
Groenewoud, Arwin
Casadei, Roberto
Recine, Federica
Miserocchi, Giacomo
Pieri, Federica
Liverani, Chiara
Bongiovanni, Alberto
Spadazzi, Chiara
de Vita, Alessandro
van der Pluijm, Gabri
Giorgini, Andrea
Biagini, Roberto
Amadori, Dino
Ibrahim, Toni
Snaar-Jagalska, Ewa
Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title_full Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title_fullStr Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title_full_unstemmed Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title_short Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
title_sort development of a patient-derived xenograft (pdx) of breast cancer bone metastasis in a zebrafish model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000770/
https://www.ncbi.nlm.nih.gov/pubmed/27556456
http://dx.doi.org/10.3390/ijms17081375
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