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Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock–induced oxidation
Testes of most male mammals present the particularity of being externalized from the body and are consequently slightly cooler than core body temperature (4–8°C below). Although, hypothermia of the testis is known to increase germ cells apoptosis, little is known about the underlying molecular mecha...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Federation of American Societies for Experimental
Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001517/ https://www.ncbi.nlm.nih.gov/pubmed/27317670 http://dx.doi.org/10.1096/fj.201600257R |
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author | Borowiec, Anne-Sophie Sion, Benoit Chalmel, Frédéric D. Rolland, Antoine Lemonnier, Loïc De Clerck, Tatiana Bokhobza, Alexandre Derouiche, Sandra Dewailly, Etienne Slomianny, Christian Mauduit, Claire Benahmed, Mohamed Roudbaraki, Morad Jégou, Bernard Prevarskaya, Natalia Bidaux, Gabriel |
author_facet | Borowiec, Anne-Sophie Sion, Benoit Chalmel, Frédéric D. Rolland, Antoine Lemonnier, Loïc De Clerck, Tatiana Bokhobza, Alexandre Derouiche, Sandra Dewailly, Etienne Slomianny, Christian Mauduit, Claire Benahmed, Mohamed Roudbaraki, Morad Jégou, Bernard Prevarskaya, Natalia Bidaux, Gabriel |
author_sort | Borowiec, Anne-Sophie |
collection | PubMed |
description | Testes of most male mammals present the particularity of being externalized from the body and are consequently slightly cooler than core body temperature (4–8°C below). Although, hypothermia of the testis is known to increase germ cells apoptosis, little is known about the underlying molecular mechanisms, including cold sensors, transduction pathways, and apoptosis triggers. In this study, using a functional knockout mouse model of the cold and menthol receptors, dubbed transient receptor potential melastatine 8 (TRPM8) channels, we found that TRPM8 initiated the cold-shock response by differentially modulating cold- and heat-shock proteins. Besides, apoptosis of germ cells increased in proportion to the cooling level in control mice but was independent of temperature in knockout mice. We also observed that the rate of germ cell death correlated positively with the reactive oxygen species level and negatively with the expression of the detoxifying enzymes. This result suggests that the TRPM8 sensor is a key determinant of germ cell fate under hypothermic stimulation.—Borowiec, A.-S., Sion, B., Chalmel, F., Rolland, A. D., Lemonnier, L., De Clerck, T., Bokhobza, A., Derouiche, S., Dewailly, E., Slomianny, C., Mauduit, C., Benahmed, M., Roudbaraki, M., Jégou, B., Prevarskaya, N., Bidaux, G. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock–induced oxidation. |
format | Online Article Text |
id | pubmed-5001517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Federation of American Societies for Experimental
Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-50015172016-08-30 Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock–induced oxidation Borowiec, Anne-Sophie Sion, Benoit Chalmel, Frédéric D. Rolland, Antoine Lemonnier, Loïc De Clerck, Tatiana Bokhobza, Alexandre Derouiche, Sandra Dewailly, Etienne Slomianny, Christian Mauduit, Claire Benahmed, Mohamed Roudbaraki, Morad Jégou, Bernard Prevarskaya, Natalia Bidaux, Gabriel FASEB J Research Testes of most male mammals present the particularity of being externalized from the body and are consequently slightly cooler than core body temperature (4–8°C below). Although, hypothermia of the testis is known to increase germ cells apoptosis, little is known about the underlying molecular mechanisms, including cold sensors, transduction pathways, and apoptosis triggers. In this study, using a functional knockout mouse model of the cold and menthol receptors, dubbed transient receptor potential melastatine 8 (TRPM8) channels, we found that TRPM8 initiated the cold-shock response by differentially modulating cold- and heat-shock proteins. Besides, apoptosis of germ cells increased in proportion to the cooling level in control mice but was independent of temperature in knockout mice. We also observed that the rate of germ cell death correlated positively with the reactive oxygen species level and negatively with the expression of the detoxifying enzymes. This result suggests that the TRPM8 sensor is a key determinant of germ cell fate under hypothermic stimulation.—Borowiec, A.-S., Sion, B., Chalmel, F., Rolland, A. D., Lemonnier, L., De Clerck, T., Bokhobza, A., Derouiche, S., Dewailly, E., Slomianny, C., Mauduit, C., Benahmed, M., Roudbaraki, M., Jégou, B., Prevarskaya, N., Bidaux, G. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock–induced oxidation. Federation of American Societies for Experimental Biology 2016-09 2016-06-17 /pmc/articles/PMC5001517/ /pubmed/27317670 http://dx.doi.org/10.1096/fj.201600257R Text en © The Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (http://creativecommons.org/licenses/by-nc/4.0/) which permits noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Borowiec, Anne-Sophie Sion, Benoit Chalmel, Frédéric D. Rolland, Antoine Lemonnier, Loïc De Clerck, Tatiana Bokhobza, Alexandre Derouiche, Sandra Dewailly, Etienne Slomianny, Christian Mauduit, Claire Benahmed, Mohamed Roudbaraki, Morad Jégou, Bernard Prevarskaya, Natalia Bidaux, Gabriel Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock–induced oxidation |
title | Cold/menthol TRPM8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
title_full | Cold/menthol TRPM8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
title_fullStr | Cold/menthol TRPM8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
title_full_unstemmed | Cold/menthol TRPM8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
title_short | Cold/menthol TRPM8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
title_sort | cold/menthol trpm8 receptors initiate the cold-shock response and protect
germ cells from cold-shock–induced oxidation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001517/ https://www.ncbi.nlm.nih.gov/pubmed/27317670 http://dx.doi.org/10.1096/fj.201600257R |
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