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Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001591/ https://www.ncbi.nlm.nih.gov/pubmed/27166377 http://dx.doi.org/10.1093/nar/gkw404 |
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author | Hao, Yue Updegrove, Taylor B. Livingston, Natasha N. Storz, Gisela |
author_facet | Hao, Yue Updegrove, Taylor B. Livingston, Natasha N. Storz, Gisela |
author_sort | Hao, Yue |
collection | PubMed |
description | Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same terminator. Low temperature, rich nitrogen sources and the Crl and NarP transcription factors differentially affect the levels of the SdsN transcripts. Whole genome expression analysis after pulse overexpression of SdsN(137) and assays of lacZ fusions revealed that the SdsN(137) directly represses the synthesis of the nitroreductase NfsA, which catalyzes the reduction of the nitrogroup (NO(2)) in nitroaromatic compounds and the flavohemoglobin HmpA, which has aerobic nitric oxide (NO) dioxygenase activity. Consistent with this regulation, SdsN(137) confers resistance to nitrofurans. In addition, SdsN(137) negatively regulates synthesis of NarP. Interestingly, SdsN(178) is defective at regulating the above targets due to unusual binding to the Hfq protein, but cleavage leads to a shorter form, SdsN(124), able to repress nfsA and hmpA. |
format | Online Article Text |
id | pubmed-5001591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50015912016-12-07 Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA Hao, Yue Updegrove, Taylor B. Livingston, Natasha N. Storz, Gisela Nucleic Acids Res RNA Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same terminator. Low temperature, rich nitrogen sources and the Crl and NarP transcription factors differentially affect the levels of the SdsN transcripts. Whole genome expression analysis after pulse overexpression of SdsN(137) and assays of lacZ fusions revealed that the SdsN(137) directly represses the synthesis of the nitroreductase NfsA, which catalyzes the reduction of the nitrogroup (NO(2)) in nitroaromatic compounds and the flavohemoglobin HmpA, which has aerobic nitric oxide (NO) dioxygenase activity. Consistent with this regulation, SdsN(137) confers resistance to nitrofurans. In addition, SdsN(137) negatively regulates synthesis of NarP. Interestingly, SdsN(178) is defective at regulating the above targets due to unusual binding to the Hfq protein, but cleavage leads to a shorter form, SdsN(124), able to repress nfsA and hmpA. Oxford University Press 2016-08-19 2016-05-10 /pmc/articles/PMC5001591/ /pubmed/27166377 http://dx.doi.org/10.1093/nar/gkw404 Text en Published by Oxford University Press on behalf of Nucleic Acids Research 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US. |
spellingShingle | RNA Hao, Yue Updegrove, Taylor B. Livingston, Natasha N. Storz, Gisela Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title | Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title_full | Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title_fullStr | Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title_full_unstemmed | Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title_short | Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA |
title_sort | protection against deleterious nitrogen compounds: role of σ(s)-dependent small rnas encoded adjacent to sdia |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001591/ https://www.ncbi.nlm.nih.gov/pubmed/27166377 http://dx.doi.org/10.1093/nar/gkw404 |
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