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Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA

Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same...

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Detalles Bibliográficos
Autores principales: Hao, Yue, Updegrove, Taylor B., Livingston, Natasha N., Storz, Gisela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001591/
https://www.ncbi.nlm.nih.gov/pubmed/27166377
http://dx.doi.org/10.1093/nar/gkw404
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author Hao, Yue
Updegrove, Taylor B.
Livingston, Natasha N.
Storz, Gisela
author_facet Hao, Yue
Updegrove, Taylor B.
Livingston, Natasha N.
Storz, Gisela
author_sort Hao, Yue
collection PubMed
description Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same terminator. Low temperature, rich nitrogen sources and the Crl and NarP transcription factors differentially affect the levels of the SdsN transcripts. Whole genome expression analysis after pulse overexpression of SdsN(137) and assays of lacZ fusions revealed that the SdsN(137) directly represses the synthesis of the nitroreductase NfsA, which catalyzes the reduction of the nitrogroup (NO(2)) in nitroaromatic compounds and the flavohemoglobin HmpA, which has aerobic nitric oxide (NO) dioxygenase activity. Consistent with this regulation, SdsN(137) confers resistance to nitrofurans. In addition, SdsN(137) negatively regulates synthesis of NarP. Interestingly, SdsN(178) is defective at regulating the above targets due to unusual binding to the Hfq protein, but cleavage leads to a shorter form, SdsN(124), able to repress nfsA and hmpA.
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spelling pubmed-50015912016-12-07 Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA Hao, Yue Updegrove, Taylor B. Livingston, Natasha N. Storz, Gisela Nucleic Acids Res RNA Here, we report the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA. Two longer sRNAs, SdsN(137) and SdsN(178) are transcribed from two σ(S)-dependent promoters but share the same terminator. Low temperature, rich nitrogen sources and the Crl and NarP transcription factors differentially affect the levels of the SdsN transcripts. Whole genome expression analysis after pulse overexpression of SdsN(137) and assays of lacZ fusions revealed that the SdsN(137) directly represses the synthesis of the nitroreductase NfsA, which catalyzes the reduction of the nitrogroup (NO(2)) in nitroaromatic compounds and the flavohemoglobin HmpA, which has aerobic nitric oxide (NO) dioxygenase activity. Consistent with this regulation, SdsN(137) confers resistance to nitrofurans. In addition, SdsN(137) negatively regulates synthesis of NarP. Interestingly, SdsN(178) is defective at regulating the above targets due to unusual binding to the Hfq protein, but cleavage leads to a shorter form, SdsN(124), able to repress nfsA and hmpA. Oxford University Press 2016-08-19 2016-05-10 /pmc/articles/PMC5001591/ /pubmed/27166377 http://dx.doi.org/10.1093/nar/gkw404 Text en Published by Oxford University Press on behalf of Nucleic Acids Research 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.
spellingShingle RNA
Hao, Yue
Updegrove, Taylor B.
Livingston, Natasha N.
Storz, Gisela
Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title_full Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title_fullStr Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title_full_unstemmed Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title_short Protection against deleterious nitrogen compounds: role of σ(S)-dependent small RNAs encoded adjacent to sdiA
title_sort protection against deleterious nitrogen compounds: role of σ(s)-dependent small rnas encoded adjacent to sdia
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001591/
https://www.ncbi.nlm.nih.gov/pubmed/27166377
http://dx.doi.org/10.1093/nar/gkw404
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