Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin

We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proxi...

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Detalles Bibliográficos
Autores principales: Baker, Christopher A, Elyada, Yishai M, Parra, Andres, Bolton, M McLean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001837/
https://www.ncbi.nlm.nih.gov/pubmed/27525487
http://dx.doi.org/10.7554/eLife.14193
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author Baker, Christopher A
Elyada, Yishai M
Parra, Andres
Bolton, M McLean
author_facet Baker, Christopher A
Elyada, Yishai M
Parra, Andres
Bolton, M McLean
author_sort Baker, Christopher A
collection PubMed
description We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording. DOI: http://dx.doi.org/10.7554/eLife.14193.001
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spelling pubmed-50018372016-08-29 Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin Baker, Christopher A Elyada, Yishai M Parra, Andres Bolton, M McLean eLife Neuroscience We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording. DOI: http://dx.doi.org/10.7554/eLife.14193.001 eLife Sciences Publications, Ltd 2016-08-15 /pmc/articles/PMC5001837/ /pubmed/27525487 http://dx.doi.org/10.7554/eLife.14193 Text en © 2016, Baker et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Baker, Christopher A
Elyada, Yishai M
Parra, Andres
Bolton, M McLean
Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title_full Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title_fullStr Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title_full_unstemmed Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title_short Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
title_sort cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001837/
https://www.ncbi.nlm.nih.gov/pubmed/27525487
http://dx.doi.org/10.7554/eLife.14193
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