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Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin
We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proxi...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
eLife Sciences Publications, Ltd
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001837/ https://www.ncbi.nlm.nih.gov/pubmed/27525487 http://dx.doi.org/10.7554/eLife.14193 |